AMP Colorimetric Assay Kit

Adenosine Monophosphate (AMP), also known as 5′-adenylic acid, consists of adenine, ribose and phosphate. AMP can be produced during ATP synthesis by the enzyme adenylate kinase by combining two ADP molecules, as a result of the hydrolysis of ATP/ADP, or when RNA is broken down. AMP plays an important role in many cellular metabolic processes, such as Ca²⁺ signaling, cell migration, cytokine secretion etc. AMP is an activator of AMP-activated protein kinase, which regulates glucose uptake, fatty acid uptake, fatty acid oxidation etc. AMP levels can be measured by luciferase/luciferin mediated assays. However, luciferase signal is unstable and luminescent equipment is generally not available in most laboratories.

AMP Assay Kit:

• Compatible with high-throughput handling systems.
• Sensitive, detecting AMP levels lower than 10 μM in various sample types.

The kit is suitable for the measurement of AMP levels in various cell (adherent or suspension cells: e.g. HeLa, HEK239, Jurkat cells) and tissue lysates (e.g. liver, kidney, etc.), the study of the regulation of AMPK by AMP and for the mechanistic study of key cellular processes such as Ca²⁺ signaling, glucose uptake, lipid uptake, etc.

In this assay, AMP is converted to pyruvate in the presence of pyrophosphate and phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at A₅₇₀ nm. The absorbance is proportional to the amount of AMP present in samples.