Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric)

The new Nitric Oxide Synthase (NOS) Assay Kit, MAK532, is now available! Nitric oxide synthases (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In the presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in the central nervous system, where NO participates in cell communication and information storage. eNOS produces NO in blood vessels and is involved in regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as part of the body’s defense mechanism.

Nitric Oxide Synthase (NOS) Activity Assay Kit:

• Compatible with high-throughput handling systems.
• Sensitive, detecting as low as 5 μU of NOS activity.

The kit is suitable for the detection of NOS activity in purified recombinant protein and tissue or cell extracts.

In this assay, nitric oxide generated by NOS undergoes a series of reactions and reacts with Griess Reagent 1 and 2 to generate a colored product with a strong absorbance at 540 nm