MAK401
Cathepsin L Activity Assay Kit
sufficient for 100 fluorometric tests
detection method
fluorometric
relevant disease(s)
cancer; cardiovascular diseases
storage temp.
−20°C
General description
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
Application
The kit is suitable for the measurement of cathepsin L in cell lysates.
Biochem/physiol Actions
The Cathepsin L Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin L substrate sequence FR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin L will cleave the synthetic substrate FR-AFC to release free AFC. The released AFC can easily be quantified fluorometrically. The cathepsin L assay is simple and straightforward. Assay conditions have been optimized to obtain the maximal activity.
Features and Benefits
Cathepsin L Activity Assay Kit:
- Simple procedure with results in 1-2 hours.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
wgk
WGK 3
Regulatory Information
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