α-Glucosidase Activity Colorimetric Assay Kit

α-Glucosidase breaks down α-1,4 linked polysaccharides to glucose, which can be utilized as a source of energy. In the biotechnology industry, α-glucosidase is used to produce glucose from intermediate breakdown products of starch hydrolysis generated by enzymes such as amylase. Pompe disease, one of the 12 known glycogen storage diseases, is an autosomal recessive metabolic disorder attributed to α-glucosidase deficiency. In this disease, glycogen accumulates in the lysosomes, resulting in progressive muscle weakness, heart failure and other neurological symptoms.

α-Glucosidase Activity Colorimetric Assay Kit:

• Compatible with high-throughput handling systems.
• Measures 0.1-10 mU of α-glucosidase.

The kit is suitable for the determination of α-glucosidase activity in a variety of samples including serum, saliva, tissue and cell culture.

In the α-Glucosidase Activity Colorimetric Assay Kit, α-glucosidase hydrolyzes the substrate mix to release the p-nitrophenol that can be measured colorimetrically (A₄₁₀ nm).