MAK319
Isocitrate Assay Kit
sufficient for 100 fluorometric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
application(s)
cosmetics
food and beverages
detection method
fluorometric
storage temp.
−20°C
General description
Isocitrate (Isocitric Acid) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the quantitative determination of isocitrate and evaluation of drug effects on its metabolism in food, beverage and biological samples (e.g. cell lysate, tissue homogenate, serum, etc.).
Principle
This Isocitrate Assay Kit measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at ex = 530 nm/ em = 585 nm, is proportional to the isocitrate concentration in the sample.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Ya-Nan Xue et al.
Cancer medicine, 8(5), 2462-2473 (2019-04-12)
Metabolic reprogramming is a central hallmark of cancer. Therefore, targeting metabolism may provide an effective strategy for identifying promising drug targets for cancer treatment. In prostate cancer, cells undergo metabolic transformation from zinc-accumulating, citrate-producing cells to citrate-oxidizing malignant cells with
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