MAK265
Fructose Colorimetric/Fluorometric Assay Kit
sufficient for 100 colorimetric or fluorometric tests
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Synonym(s):
Fructose Detection Kit
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
detection method
colorimetric
fluorometric
storage temp.
−20°C
General description
Fructose is a monosaccharide naturally found in honey and fruits. Several studies have linked fructose to the development of the metabolic syndrome particularly through promoting insulin resistance in the liver. It has also been implicated in the obesity epidemic. Studies in animals have revealed high fructose intake during pregnancy and lactation may lead to metabolic dysfunctions in the mother and the newborn. Fructose measurements can provide useful insights into metabolic and biochemical functions. Excessive uptake of fructose is implicated in obesity in children. Fructose induced hyperuricemia, reduces nitric oxide bioavailability for insulin and thus it is implicated in diabetes. Fructose might be implicated in cardiovascular disease, as it is the only sugar that raises uric acid production in the body. High fructose diet is known to induce hypertension.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for detecting fructose in cell and tissue culture supernatants. Note: Not suitable for urine, plasma, or serum.
Principle
In this assay kit, fructose is converted to -glucose resulting in a colorimetric (570 nm)/fluorometric (ex = 535 nm/em = 587 nm) product proportional to fructose present. This kit has a linear range of detection between 0.2–1.0 nmole fructose for the fluorometric assay and 2–10 nmole fructose for the colorimetric assay.
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1
WGK
WGK 3
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Potential role of sugar (fructose) in the epidemic of hypertension, obesity and the metabolic syndrome, diabetes, kidney disease, and cardiovascular disease.
Johnson R J, et al.
American Journal of Clinical Nutrition, 86(4), 899-906 (2007)
A causal role for uric acid in fructose-induced metabolic syndrome.
Nakagawa T, et al.
American Journal of Physiology: Renal Physiology, 290(3), F625-F631 (2006)
Nattawan Suwannakul et al.
Genes & diseases, 9(6), 1727-1741 (2022-09-27)
Alterations in cellular metabolism may contribute to tumor proliferation and survival. Upregulation of the facilitative glucose transporter (GLUT) plays a key role in promoting cancer. GLUT5 mediates modulation of fructose utilization, and its overexpression has been associated with poor prognosis
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