Adenylate Kinase Activity Assay Kit (Colorimetric/Fluorometric)

Adenylate Kinase (AK) (EC 2.7.4.3) is an abundant enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. The enzyme is found in the nucleus, cytosol, or mitochondria (intermembrane space or matrix) of various kinds of tissues. Adenylate kinase acts on two molecules of ADP to generate ATP and AMP. Nine isoforms of adenylate kinase have been identified. Erythrocyte adenylate kinase deficiency is associated with hemolytic anemia. Adenylate kinase also plays an important role in post-ischemic recovery and in apoptosis.
About 30 – 40% of adenylate kinase activity takes place in mitochondria of heart muscles. Mutation in adenylate kinase gene is associated with a severe combined immunodeficiency referred as reticular dysgenesis.

Suitable for the detection of Adenylate Kinase in cell/tissue homogenate, mitochondrial lysate, or purified protein.

This AK Activity Assay kit can kinetically measure Adenylate Kinase activity by detecting adenosine triphosphate (ATP) generated from adenosine diphosphate (ADP) as a substrate. ATP is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the Adenylate Kinase Probe forming a colorimetric (570 nm)/fluorometric (ex = 535 nm/em = 587 nm) product.