MAK228
Calpain Activity Fluorometric Assay Kit
sufficient for 100 fluorometric tests
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About This Item
UNSPSC Code:
12352200
detection method
fluorometric
relevant disease(s)
endocrinological disorders, diabetes; neurological disorders; cancer; cardiovascular diseases
storage temp.
−70°C
General description
Activation of calpain is involved in many forms of physiological and pathological processes (e.g., apoptosis). Calpain activation requires cell membrane and Ca2+, and activated calpain is released into cytosol. The Calpain Activity Assay Kit provides optimized buffers and reagents for a convenient measurement of calpain activity.
Suitability
Suitable for analyzing calpain activity in apoptotic and other samples.
Principle
Calpain Activity Fluorometric Assay Kit is based on the cleavage of the calpain substrate, Ac-LLY-AFC. The Ac-LLY-AFC substrate emits blue light (max = 400 nm); upon cleavage by calpain, free AFC emits a yellow-green fluorescence ( max = 505 nm), which can be quantified using a fluorometer or a fluorescence plate reader. Comparison of the fluorescence intensity from a treated sample with a normal control allows determination of the changes in calpain activity.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
10 - Combustible liquids
Regulatory Information
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Certificates of Analysis (COA)
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