MAK215
High Sensitivity Lipolysis Assay Kit
Sufficient for 100 Fluorometric tests
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About This Item
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detection method
fluorometric
storage temp.
−20°C
General description
Lipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol. This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase. Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis are associated with obesity, diabetes, and metabolic syndromes.
Suitability
Highly sensitive kit suitable for the measurement of lipolysis in 3T3-L1 and adipocytes.
Principle
The High Sensitivity Lipolysis (3T3-L1) Fluorometric Assay Kit contains synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This results in the activation of adenylate cyclase that converts ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a fluorescent product (λex = 535/ λem = 587 nm) proportional to the amount of glycerol present.
Kit Components Only
Product No.
Description
- Lipolysis Assay Buffer
- Lipolysis Wash Buffer
- Glycerol Assay Buffer
- High-Sensitivity Probe, in DMSO
- Glycerol Enzyme Mix
- Glycerol Developer
- Glycerol Standard, 100 mM
- Isoproterenol, 10 mM
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Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
10 - Combustible liquids
Regulatory Information
监管及禁止进口产品
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Qingrui Zhuan et al.
Reproduction, fertility, and development, 32(7), 667-675 (2020-03-17)
Intracellular lipids provide energy for oocyte maturation and development. Triglycerides are the main components of cytoplasm lipid droplets, and hydrolysis of triglycerides requires several lipase-mediated steps. The aim of this study was to determine the effects of the β-adrenoceptor agonist
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