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MAK214

Sigma-Aldrich

Oxalate Decarboxylase Activity Assay Kit

Sufficient for 100 Colorimetric tests

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Synonym(s):
OXDC Activity Assay Kit
EC Number:
UNSPSC Code:
12352200
NACRES:
NA.84

detection method

colorimetric

storage temp.

−20°C

General description

Oxalate Decarboxylase (OXDC, EC 4.1.1.2) catalyzes the conversion of oxalate to formate and carbon dioxide in the presence of molecular oxygen. OXDC-responsive proteins are involved in the regulation of metabolism and stress responses in plants.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of Oxalate Decarboxylase activity in samples such as plants and fungi.

Principle

The Oxalate Decarboxylase Activity Assay Kit provides a simple and high throughput adaptable procedure for measuring OXDC activity in a variety of plant and fungal tissues. OXDC activity is determined by generating a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of oxalate decarboxylase is the amount of enzyme required to generate 1.0 μmole of formate per minute at pH 5 at 37 °C.

Kit Components Only

Product No.
Description

  • OXDC Assay Buffer I

  • OXDC Assay Buffer II

  • OXDC Substrate

  • OXDC Enzyme Mix

  • OXDC Probe

  • OXDC Positive Control

  • Formate Standard, 100 mM

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

WGK

WGK 3

Regulatory Information

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