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MAK207

Sigma-Aldrich

Glycerol-3-Phosphate Assay Kit

Sufficient for 100 Colorimetric tests

Synonym(s):

G3P Assay Kit

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About This Item

EC Number:
UNSPSC Code:
12161503
NACRES:
NA.84

detection method

colorimetric

relevant disease(s)

gastrointestinal diseases

storage temp.

−20°C

General description

Glycerol 3-phosphate (G3P) is an important intermediate of carbohydrate and lipid metabolic pathways. It is produced from glycerol by glycerol kinase or from dihydroxyacetone phosphate by glycerol 3-phosphate dehydrogenase. G3P may enter the G3P shuttle to generate NAD+, or may be converted to glyceraldehyde 3-phosphate and enter glycolysis or the lipid biosynthesis pathway.

Application

Glycerol-3-Phosphate Assay Kit has been used in the determination of glycerol-3-phosphate and phosphoenolpyruvate levels. It has also been used to quantify the concentration in aerial mycelium.

Suitability

Suitable for the detection of Glycerol-3-Phosphate in tissue and cell culture samples.

Principle

The Glycerol 3-Phosphate Colorimetric Assay Kit provides a simple assay for measuring G3P in various tissues and cells (ranging from 2-10 nmole/well). G3P is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the amount of G3P present.

Kit Components Only

Product No.
Description

  • G3P Assay Buffer

  • G3P Enzyme Mix

  • G3P Probe

  • G3P Standard

replaced by

Pictograms

Health hazardCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

Regulatory Information

动植物源性产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yongkai Shi et al.
Frontiers in plant science, 9, 687-687 (2018-06-08)
The glycerol-3-phosphate (G-3-P) shuttle is an important pathway for delivery of cytosolic reducing equivalents into mitochondrial oxidative phosphorylation, and plays essential physiological roles in yeast, plants, and animals. However, its role has been unclear in filamentous and pathogenic fungi. Here
Michaela Veliova et al.
EMBO reports, 21(12), e49634-e49634 (2020-12-05)
Combined fatty acid esterification and lipolysis, termed lipid cycling, is an ATP-consuming process that contributes to energy expenditure. Therefore, interventions that stimulate energy expenditure through lipid cycling are of great interest. Here we find that pharmacological and genetic inhibition of
Alejandro Cabezas-Cruz et al.
Frontiers in cellular and infection microbiology, 7, 375-375 (2017-09-02)
The obligate intracellular pathogen, Anaplasma phagocytophilum, is the causative agent of life-threatening diseases in humans and animals. A. phagocytophilum is an emerging tick-borne pathogen in the United States, Europe, Africa and Asia, with increasing numbers of infected people and animals
Agustín Hidalgo-Gutiérrez et al.
Biomedicines, 9(10) (2021-10-24)
Primary mitochondrial diseases are caused by mutations in mitochondrial or nuclear genes, leading to the abnormal function of specific mitochondrial pathways. Mitochondrial dysfunction is also a secondary event in more common pathophysiological conditions, such as obesity and metabolic syndrome. In
Laura Di Magno et al.
Cell reports, 30(6), 1735-1752 (2020-02-13)
The antidiabetic drug phenformin displays potent anticancer activity in different tumors, but its mechanism of action remains elusive. Using Shh medulloblastoma as model, we show here that at clinically relevant concentrations, phenformin elicits a significant therapeutic effect through a redox-dependent

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