MAK206
Fumarase Activity Colorimetric Assay Kit
Sufficient for 100 Colorimetric tests
Sign Into View Organizational & Contract Pricing
All Photos(2)
Synonym(s):
Fumarate Hydratase Activity Colorimetric Assay Kit
UNSPSC Code:
12352200
NACRES:
NA.84
Recommended Products
General description
The new Fumarase Assay Kit, MAK521, is now available! Fumarase (Fumarate Hydratase; EC 4.2.1.2) catalyzes the reversible hydration of fumarate to malate in the tricarboxylic acid (Krebs) cycle. The activity of fumarase is important as it produces reducing equivalents that drive the synthesis of ATP and maintains energy balance in aerobic metabolism. A deficiency of fumarase activity results in progressive encephalopathy.
Application
Fumarase Activity Colorimetric Assay Kit has been used to determine fumarase (FUM) activity.
Suitability
Suitable for the detection of Fumarase in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.
Principle
The Fumarase Activity Colorimetric Assay Kit provides a simple and high-throughput ready procedure for measuring fumarase activity in a variety of tissues, cells, and isolated mitochondria. Fumarase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of fumarase is the amount of enzyme that generates 1.0 mmole of NADH per minute at pH 9.5 at 37 °C.
Kit Components Only
Product No.
Description
- Fumarase Assay Buffer
- Fumarase Substrate
- Fumarase Enzyme Mix
- Fumarase Developer
- NADH Standard
- Fumarase Positive Control
replaced by
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
WGK
WGK 3
Regulatory Information
监管及禁止进口产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Dieuwertje Augustijn et al.
PloS one, 14(6), e0218219-e0218219 (2019-06-27)
Climate change and the rising food demand provide a need for smart crops that yield more biomass. Recently, two Arabidopsis thaliana mutants with enhanced growth characteristics, VP16-02-003 and the VP16-05-014, were obtained by genome-wide reprogramming of gene expression, which led
Changes in specific protein degradation rates in Arabidopsis thaliana reveal multiple roles of Lon1 in mitochondrial protein homeostasis.
Li L, et al.
The Plant Journal, 89(3), 458-471 (2017)
Lei Li et al.
The Plant journal : for cell and molecular biology, 89(3), 458-471 (2016-10-12)
Mitochondrial Lon1 loss impairs oxidative phosphorylation complexes and TCA enzymes and causes accumulation of specific mitochondrial proteins. Analysis of over 400 mitochondrial protein degradation rates using 15 N labelling showed that 205 were significantly different between wild type (WT) and
Ping Li et al.
International journal of molecular sciences, 23(3) (2022-02-16)
The FH gene encodes the fumarate hydratase of the Krebs cycle and functions as a homotetramer to catalyze the hydration of fumarate to malate. Mutations in FH result in uterine leiomyomas, a rare autosomal dominant inherited metabolic disease. However, how
Helena Hernández-Cuervo et al.
Frontiers in physiology, 13, 814510-814510 (2022-04-19)
Acute Lung Injury (ALI), characterized by bilateral pulmonary infiltrates that restrict gas exchange, leads to respiratory failure. It is caused by an innate immune response with white blood cell infiltration of the lungs, release of cytokines, an increase in reactive
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service