MAK204
Enteropeptidase Activity Assay Kit
Sufficient for 100 Fluorometric tests
Synonym(s):
Enterokinase Activity Assay Kit
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About This Item
UNSPSC Code:
12352200
EC Number:
200-664-3
NACRES:
NA.84
detection method
fluorometric
storage temp.
−20°C
General description
Enteropeptidase (Enterokinase; EC 3.4.21.9) is an enzyme that converts trypsinogen to trypsin, the activity of which is required for the activation of chymotrypsin and procarboxypeptidases. It has an important role in food digestion and may serve as a marker of differentiated enterocytes and goblet cells.
Application
Suitable for the measurement of enteropeptidase/enterokinase activity in biological samples or purified preparations.
Biochem/physiol Actions
The Enteropeptidase/Enterokinase Activity Assay Kit is a rapid method to measure enteropeptidase activity in biological samples. Enteropeptidase activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-tagged peptide substrate containing the recognition sequence for enteropeptidase. This yields AFC, a fluorescent product (λex = 380/ λem = 500 nm), proportional to the enzymatic activity present. One unit of enteropeptidase is the amount of enzyme that generates 1.0 nmole of AFC per minute at room temperature.
Kit Components Only
Product No.
Description
- Enteropeptidase Assay Buffer
- Enteropeptidase Substrate, 10 mM in DMSO
- Human Enteropeptidase
- AFC Standard, 1 mM
Storage Class
10 - Combustible liquids
wgk
WGK 2
Regulatory Information
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