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MAK194

Sigma-Aldrich

Lipid (Oil Red O) Staining Kit

Sufficient for 2 96-well plates

Synonym(s):

Oil Red O Lipid Staining Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.25

relevant disease(s)

endocrinological disorders, diabetes; obesity; cardiovascular diseases

storage temp.

room temp

General description

Lipid droplets (LDs) are dynamic, ubiquitously present lipid-storage organelles, predominantly present in the adipocytes. Triglycerides, neutral lipids, and cholesterol esters stored in LDs are the largest sources of energy. The presence of excess LDs in adipocytes results in obesity and obesity-linked pathologies such as dyslipidemia and diabetes type.

Application

Lipid (Oil Red O) Staining Kit has been used to stain lipids and adipocytes in samples.

Suitability

This kit is suitable for staining neutral lipids in two 96 well plates, two 6 well plates, or four 100 mm culture dishes.

Principle

The Lipid (Oil Red O) Staining Kit is suitable for selective staining and detection of neutral lipids in cultured cells. Hematoxylin included in the kit stains the nuclei of the cells.

Kit Components Only

Product No.
Description
  • PBS
  • Formalin (10%)
  • Oil Red O
  • Hematoxylin

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Aldehyde dehydrogenase 1 a1 regulates energy metabolism in adipocytes from different species.
Yang K, et al.
Xenotransplantation, 24(5), e12318-e12318 (2017)
Yohei Nomura et al.
Frontiers in physiology, 12, 675724-675724 (2021-07-06)
We previously reported that histone deacetylase 6 (HDAC6) has an important role in endothelial cell (EC) function in vitro. However, whether HDAC6 plays a role in atherogenesis in vivo and the mechanism(s) that control HDAC6 activity/expression in response to atherogenic
CD36 mediates lipid accumulation in pancreatic beta cells under the duress of glucolipotoxic conditions: Novel roles of lysine deacetylases.
Khan S and Kowluru A
Biochemical and Biophysical Research Communications, 495(3), 2221-2226 (2018)
Kefeng Yang et al.
Xenotransplantation, 24(5) (2017-07-19)
Survival and longevity of xenotransplants depend on immune function and ability to integrate energy metabolism between cells from different species. However, mechanisms for interspecies cross talk in energy metabolism are not well understood. White adipose tissue stores energy and is
Nobuyuki Tanaka et al.
PloS one, 12(4), e0173647-e0173647 (2017-04-06)
The geometrical confinement of small cell colonies gives differential cues to cells sitting at the periphery versus the core. To utilize this effect, for example to create spatially graded differentiation patterns of human mesenchymal stem cells (hMSCs) in vitro or
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