MAK171
Colorimetric Biotin Assay Kit
sufficient for 200 colorimetric assays
Synonym(s):
Biotin Quantitation Kit
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About This Item
detection method
colorimetric
relevant disease(s)
dermatological diseases; neurological disorders
shipped in
dry ice
storage temp.
−20°C
Related Categories
General description
Biotinylation is the process of labelling a molecule with a biotin molecule to take advantage of the strong binding and high specificity of biotin and streptavidin for purification or detection applications. Biotinylation is typically used to label proteins or nucleic acid based targets for these applications. Quantitation of biotinylation can be used to optimize the labelling of a given target. Levels of biotinylation can effect detection sensitivity or functionality of a purification product.
Suitability
Suitable for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution
Principle
This Colorimetric Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay utilizes HABA (4′-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm.
Preparation Note
Storage and Stability: The kit is shipped on dry ice and storage at –20 °C, protected from light, is recommended.
Precautions and Disclaimer: This product is for R&D;use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Precautions and Disclaimer: This product is for R&D;use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Storage Class Code
10 - Combustible liquids
Regulatory Information
动植物源性产品
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Site-specific biotinylation of purified proteins using BirA.
Fairhead M and Mark H
Site-Specific Protein Labeling, 171-184 (2015)
High-throughput biotinylation of proteins.
High Throughput Protein Expression and Purification, 185-198 (2009)
Imre Gaspar et al.
Bio-protocol, 8(5), e2750-e2750 (2018-03-05)
Arrays of short, singly-labeled ssDNA oligonucleotides enable in situ hybridization with single molecule sensitivity and efficient transcript specific RNA capture. Here, we describe a simple, enzymatic protocol that can be carried out using basic laboratory equipment to convert arrays of
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