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MAK169

Sigma-Aldrich

High Sensitivity Pyrophosphate Assay Kit

sufficient for 200 fluorometric tests

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Synonym(s):
Pyrophosphate (PPi) Assay Kit
EC Number:
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 200 fluorometric tests

application(s)

cosmetics
food and beverages

detection method

fluorometric

relevant disease(s)

genitourinary disorders; rheumatological diseases

storage temp.

−20°C

General description

Pyrophosphate (PPi) is produced by a number of biochemical reactions such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters.

Suitability

The Pyrophosphate Assay Kit provides a simple and direct flourescent microplate procedure for measuring pyrophosphate in a variety of samples.

Principle

The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λex =370/λem = 470 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

WGK

WGK 3


Certificates of Analysis (COA)

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