MAK164
Intracellular Hydrogen Peroxide Assay
sufficient for 200 fluorometric tests (green fluorescence)
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Synonym(s):
Hydrogen Peroxide Detection in Cells
Recommended Products
usage
sufficient for 200 fluorometric tests (green fluorescence)
detection method
fluorometric
relevant disease(s)
aging/geriatric diseases; neurological disorders; pulmonary disorders; orthopedic diseases; cardiovascular diseases
storage temp.
−20°C
Related Categories
General description
Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.
Application
Intracellular hydrogen peroxide assay kit has been used to measure intracellular hydrogen peroxide levels.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
This kit is suitable for the detection of hydrogen peroxide in living cells and a variety of samples such as cell extracts and liquids.
Principle
The Intracellular Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in living cells and in a variety of other samples such as cellular extracts. This kit utilizes a unique cell-permeable sensor that generates a fluorescent product (λex = 490/λem = 520 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader, flow cytometer, or fluorescent microscope.
WGK
WGK 3
Certificates of Analysis (COA)
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Emily A Teslow et al.
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Carmen Sarcinelli et al.
Archaea (Vancouver, B.C.), 2016, 7424870-7424870 (2016-10-19)
Peroxiredoxins (Prxs) are ubiquitous thiol peroxidases that are involved in the reduction of peroxides. It has been reported that prokaryotic Prxs generally show greater structural robustness than their eukaryotic counterparts, making them less prone to inactivation by overoxidation. This difference
Qing You et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(17), 1903341-1903341 (2020-10-01)
Multifunctional nanoplatforms for imaging-guided synergistic antitumor treatment are highly desirable in biomedical applications. However, anticancer treatment is largely affected by the pre-existing hypoxic tumor microenvironment (TME), which not only causes the resistance of the tumors to photodynamic therapy (PDT), but
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