MAK138
Autophagy Assay Kit
sufficient for 200 fluorometric tests
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UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 200 fluorometric tests
detection method
fluorometric
relevant disease(s)
cancer; immunological diseases
storage temp.
−20°C
Related Categories
General description
Autophagy is an evolutionarily conserved degradation process that targets long-lived proteins, organelles, and other cytoplasmic components for degradation via the lysosomal pathway. Activation of the autophagy pathway is required for multiple cellular roles, including survival during starvation, the clearance of intracellular components, development, and immunity.
Application
- Inhibitory Effect of Ursolic Acid on Proliferation and Migration of Renal Carcinoma Cells and Its Mechanism.: This study explores the inhibitory effects of ursolic acid on the proliferation and migration of renal carcinoma cells. The use of an autophagy assay kit was instrumental in demonstrating that ursolic acid induces autophagy, leading to decreased cell viability and migration. These findings highlight the potential of ursolic acid as a therapeutic agent in renal carcinoma through the modulation of autophagy (Lyu et al., 2022).
Suitability
This kit is suitable for the detection of autophagy in mammalian cells by fluorescence microscopy and fluorescence microplate readers.
Principle
The Autophagy Assay kit provides a simple and direct procedure for measuring autophagy in a variety of cell types using a proprietary fluorescent autophagosome marker (λex = 333/λem = 518 nm).
WGK
WGK 2
Certificates of Analysis (COA)
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Yao Liu et al.
Experimental and therapeutic medicine, 19(6), 3723-3737 (2020-04-30)
Continuous activation of angiotensin II (Ang II) induces renal vascular endothelial dysfunction, inflammation and oxidative stress, all of which may contribute to renal damage. MicroRNAs (miRs/miRNAs) play a crucial regulatory role in the pathogenesis of hypertensive nephropathy (HN). The present
Autophagy: machinery and regulation.
Yin Z, et al.
Microbial cell, 3(12), 588-588 (2016)
Autophagy in the pathogenesis of disease.
Levine B and Guido K
Cell, 132(1), 27-42 (2008)
Simin Nazarnezhad et al.
Neurotoxicology, 74, 196-202 (2019-07-20)
Diabetic neuropathy (DN) is the most common complication of diabetes mellitus. It is thought that neuronal cell death which is mainly due to reactive oxygen species (ROS) overproduction in the cells is responsible for most symptoms of this disorder. Nesfatin-1
Platelet rich plasma (PRP) induces chondroprotection via increasing autophagy, anti-inflammatory markers, and decreasing apoptosis in human osteoarthritic cartilage.
Moussa M, et al.
Experimental Cell Research, 352(1), 146-156 (2017)
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