MAK120
Urease Activity Assay Kit
sufficient for 100 colorimetric tests
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Synonym(s):
Urea Amidohydrolase Activity Assay Kit
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric tests
application(s)
environmental
detection method
colorimetric
relevant disease(s)
gastrointestinal diseases
storage temp.
2-8°C
Related Categories
General description
Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is found in bacteria, yeast, and certain higher plant species. Urease activity is found in environmental and fecal samples.
Application
Urease Activity Assay Kit has been used to measure urease activity.
Features and Benefits
Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.
Suitability
Suitable for the detection of Urease activity in biological and environmental samples.
Principle
In this assay, urease catalyzes the hydrolysis of urea resulting in the production of ammonia. The ammonia is determined by the Berthelot method resulting in colorimetric product measure at 670 nm, proportionate to the urease activity present in the sample. One unit of urease is the amount of enzyme that catalyzes the formation of 1.0 μmole ammonia per minute at pH 7.0.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B
Supplementary Hazards
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ureases I. Functional, catalytic and kinetic properties: A review
Barbara K.
Journal of Molecular Catalysis. B, Enzymatic, 59(1-3), 9-21 (2009)
Chelsea M Heveran et al.
Scientific reports, 9(1), 14721-14721 (2019-10-13)
We demonstrate for the first time that the morphology and nanomechanical properties of calcium carbonate (CaCO3) can be tailored by modulating the precipitation kinetics of ureolytic microorganisms through genetic engineering. Many engineering applications employ microorganisms to produce CaCO3. However, control
Nitis Smanthong et al.
Molecules (Basel, Switzerland), 27(3) (2022-02-16)
Proteus mirabilis is a significant cause of urinary tract infection that may contribute to struvite stones. Anti-infection of this bacterium and anti-struvite formation must be considered. Sida acuta Burm. F. (SA) has been used for the treatment of diseases related
Malassezia furfur promoting growth of Staphylococcus epidermidis by increasing pH when cultured in a lipid-free environment.
Yang Han et al.
Chinese medical journal, 132(7), 873-876 (2019-03-22)
Short-term treatment with the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) alters urea assimilation and modulates transcriptional profiles of genes involved in primary and secondary metabolism in maize seedlings.
Zanin L, et al.
Frontiers in Plant Science, 7, 845-845 (2016)
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