MAK113
ATPase/GTPase Activity Assay Kit
sufficient for 200 colorimetric tests
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Product Name
ATPase/GTPase Activity Assay Kit, sufficient for 200 colorimetric tests
usage
sufficient for 200 colorimetric tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
technique(s)
activity assay: suitable
input
liquid
detection method
colorimetric
relevant disease(s)
cancer
storage temp.
2-8°C
Related Categories
Application
Suitable for the detection of ATPase and GTPase activity and for the screening of ATPase and GTPase inhibitors.
ATPase/GTPase Activity Assay Kit has been used to measure the ATPase enzyme activity in:
- Dicer-like 1 (DCL1) and its mutants, to terminate the reaction and generate a colorimetric product
- various brain regions of mice, facilitating the quantification of inorganic phosphate released from ATP
- cell lysates, for the development and encapsulation of the reconstituted cell-free enzyme system
Biochem/physiol Actions
The ATPase/GTPase Activity Assay kit provides a simple and direct procedure for measuring ATPase/GTPase activity in a microplate format. This kit uses a single reagent formulation to accurately determine enzyme activity in 30 minutes at room temperature. The malachite green reagent forms a stable dark green color with free phosphate liberated by the enzymes resulting in a colorimetric product, measured at 620 nm (600-660 nm), proportional to the enzyme activity present One unit of activity is the amount of enzyme that catalyzes the production of 1 μmole of free phosphate per minute under the assay conditions.
Features and Benefits
Compatible with high-throughput handling systems.
General description
ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate. These enzymes play key roles in transport, signal transduction, protein biosynthesis, and cell differentiation.
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
8B - Non-combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
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Shannon E Hill et al.
PloS one, 12(7), e0180241-e0180241 (2017-07-26)
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Shuhui Wang et al.
Protein & cell, 11(2), 124-137 (2019-11-24)
Type VII secretion systems (T7SSs) are found in many disease related bacteria including Mycobacterium tuberculosis (Mtb). ESX-1 [early secreted antigen 6 kilodaltons (ESAT-6) system 1] is one of the five subtypes (ESX-1~5) of T7SSs in Mtb, where it delivers virulence
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Scientific reports, 8(1), 9706-9706 (2018-06-28)
The resistance to sorafenib highly affects its clinical benefits for treating hepatocellular carcinoma (HCC). Sodium orthovanadate (SOV) is a phosphate analog that displays anti-cancer activities against various types of malignancies including HCC. The present study has demonstrated that SOV is
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Bioorganic & medicinal chemistry, 27(7), 1274-1282 (2019-02-23)
Filamenting temperature-sensitive mutant Z (FtsZ) is recognized as a promising target for new antibiotics development because of its high conservatism and pivotal role in the bacteria cell division. The aromatic heterocyclic scaffold of indole is known showing merit medical functions
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