MAK113
ATPase/GTPase Activity Assay Kit
sufficient for 200 colorimetric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
usage
sufficient for 200 colorimetric tests
detection method
colorimetric
relevant disease(s)
cancer
storage temp.
2-8°C
Related Categories
General description
ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate. These enzymes play key roles in transport, signal transduction, protein biosynthesis, and cell differentiation.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the detection of ATPase and GTPase activity and for the screening of ATPase and GTPase inhibitors.
Principle
The ATPase/GTPase Activity Assay kit provides a simple and direct procedure for measuring ATPase/GTPase activity in a microplate format. This kit uses a single reagent formulation to accurately determine enzyme activity in 30 minutes at room temperature. The malachite green reagent forms a stable dark green color with free phosphate liberated by the enzymes resulting in a colorimetric product, measured at 620 nm (600-660 nm), proportional to the enzyme activity present One unit of activity is the amount of enzyme that catalyzes the production of 1 μmole of free phosphate per minute under the assay conditions.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Met. Corr. 1
Storage Class Code
8B - Non-combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Meng-Yan Sun et al.
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Protein & cell, 11(2), 124-137 (2019-11-24)
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The proteasome is a key player of regulated protein degradation in all kingdoms of life. Although recent atomic structures have provided snapshots on a number of conformations, data on substrate states and populations during the active degradation process in solution
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Bioorganic & medicinal chemistry, 27(7), 1274-1282 (2019-02-23)
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