MAK103
Phosphoglucose Isomerase Colorimetric Assay Kit
sufficient for 100 colorimetric tests
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About This Item
UNSPSC Code:
12352200
Recommended Products
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
storage temp.
−20°C
General description
Phosphoglucose Isomerase (PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate in the second step of glycolysis and is involved in glucogenesis. PGI is also referred to as Autocrine Motility Factor (AMF) and is secreted by cancer cells in order to stimulate metastasis. Deficiencies in PGI activity can lead to hemolytic anemia.
Suitability
Suitable for the measurement of phosphoglucose isomerase activity, analysis of glucose metabolism and cell signaling and screening anti-diabetic drugs in animal tissues (liver, brain, heart, kidney etc.), cell culture (adherent or suspension cells), bacteria, yeast, fish etc.
Principle
The Phosphoglucose Isomerase Colorimetric Assay kit provides a simple and direct procedure for measuring PGI activity in a variety of samples. PGI activity is determined by a coupled enzyme assay in which fructose-6-phosphate is converted by PGI to glucose-6-phosphate. Glucose-6-phosphate is subsequently oxidized to form a product, which reacts with a probe generating a colorimetric (450 nm) product proportional to the PGI activity present. One unit of PGI is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at room temperature.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
Regulatory Information
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Crystal structure of rabbit phosphoglucose isomerase complexed with its substrate D-fructose 6-phosphate.
Lee J H, et al.
Biochemistry, 40(26), 7799-7805 (2001)
Seok Hyun Park et al.
Nature communications, 5, 4618-4618 (2014-08-06)
L-arginine is an important amino acid for diverse industrial and health product applications. Here we report the development of metabolically engineered Corynebacterium glutamicum ATCC 21831 for the production of L-arginine. Random mutagenesis is first performed to increase the tolerance of
Metabolic engineering of Corynebacterium glutamicum for L-arginine production.
Park S H, et al.
Nature Communications, 5, 4618-4618 (2014)
Regulation of cell proliferation by autocrine motility factor/phosphoglucose isomerase signaling.
Tsutsumi S, et al.
The Journal of Biological Chemistry, 278(34), 32165-32172 (2003)
Altered expression profile of glycolytic enzymes during testicular ischemia reperfusion injury is associated with the p53/TIGAR pathway: effect of fructose 1, 6-diphosphate.
Al-Maghrebi M and Waleed M R
PeerJ, 4, e2195-e2195 (2016)
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