MAK102
PEP Colorimetric/Fluorometric Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
Phospho(enol)pyruvate Colorimetric/Fluorometric Assay Kit
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About This Item
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usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric
fluorometric
relevant disease(s)
endocrinological disorders, diabetes; cancer
storage temp.
−20°C
General description
Phospho(enol)pyruvate (PEP) in an intermediate in carbohydrate metabolism involved in glycolysis and gluconeogeneis. In glycolysis, PEP is metabolized by pyruvate kinase to yield pyruvate. In plants, PEP is involved in the formation of aromatic amino acids as well as in the carbon fixation pathway.
Application
PEP Colorimetric/Fluorometric Assay Kit has been used to measure the concentrations of phosphoenolpyruvate.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the measurement of phosphoenolpyruvate (PEP) in tissue, cells and plant extracts
Principle
PEP concentration is determined by a coupled enzyme assay, in which PEP is converted to ATP and pyruvate, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the PEP present.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
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Desideri E, et al.
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Insights into the Autotrophic CO2 Fixation Pathway of the Archaeon Ignicoccus hospitalis: Comprehensive Analysis of the Central Carbon Metabolism.
Ulrike J, et al.
Journal of Bacteriology, 189(11), 4108-4119 (2007)
Ixodes scapularis Tick Cells Control Anaplasma phagocytophilum Infection by Increasing the Synthesis of Phosphoenolpyruvate from Tyrosine.
Alejandro C C, et al.
Frontiers in Cellular and Infection Microbiology, 7, 375-375 (2017)
The transcription factor Cabut coordinates energy metabolism and the circadian clock in response to sugar sensing.
Bartok O, et al.
The Embo Journal, 34(11), 1538-1553 (2015)
Synthesis of phosphoenol pyruvate (PEP) analogues and evaluation as inhibitors of PEP-utilizing enzymes.
Garcia A, et al.
FEBS Journal, 269(13), 3226-3236 (2002)
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