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MAK095

Sigma-Aldrich

Aspartate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

endocrinological disorders, diabetes; neurological disorders

storage temp.

−20°C

Related Categories

General description

Aspartate, the carboxylate anion of aspartic acid, is an acidic, non-essential amino acid involved in protein synthesis and multiple other cellular biochemical pathways. Aspartate contributes to nucleotide synthesis via the synthesis of the precursor inosine mono-phosphate. In the urea cycle, aspartate is a key metabolite, donating a nitrogen group towards the formation of urea. Aspartate is also critical for oxidative phosphorylation as part of the aspartate-malate shuttle, which transfers reducing equivalents across the mitochondrial membrane.

Application

Aspartate Assay Kit has been used to measure the aspartate levels in nerve cells and Lactococcus lactis strain cultured in lysogeny broth (LB) medium.

Suitability

The Aspartate Assay Kit is suitable for aspartate detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

Principle

Aspartate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the aspartate present. Typical detection ranges for this kit are 2-10 nmole (colorimetric) and 0.2-1 nmole (fluorometric).

replaced by

WGK

WGK 3

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Influence of ketamine on amino acid neurotransmitters secretion by nerve cells in vitro.
Shi M, et al.
Bangladesh Journal of Pharmacology, 11(S1), 55-60 (2016)
Role of the Malate-Aspartate Shuttle on the Metabolic Response to Myocardial Ischemia
Ming Lu, et al.
Journal of Theoretical Biology, 254(2), 466-475 (2008)
W Brian Dalton et al.
The Journal of clinical investigation, 129(11), 4708-4723 (2019-08-09)
Cancer-associated mutations in the spliceosome gene SF3B1 create a neomorphic protein that produces aberrant mRNA splicing in hundreds of genes, but the ensuing biologic and therapeutic consequences of this missplicing are not well understood. Here we have provided evidence that
Philip H Choi et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(35), E7226-E7235 (2017-08-16)
Cyclic di-3',5'-adenosine monophosphate (c-di-AMP) is a broadly conserved bacterial second messenger that has been implicated in a wide range of cellular processes. Our earlier studies showed that c-di-AMP regulates central metabolism in Listeria monocytogenes by inhibiting its pyruvate carboxylase (LmPC)
The Impact of Inflammation on Pancreatic β-Cell Metabolism, Function and Failure in T1DM and T2DM: Commonalities and Differences.
Newsholme P, et al.
Type 1 Diabetes, 125-165 (2013)

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