MAK093
Phosphofructokinase (PFK) Activity Colorimetric Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
PFK Activity Assay Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Product Name
Phosphofructokinase (PFK) Activity Colorimetric Assay Kit, sufficient for 100 colorimetric tests
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
cancer; orthopedic diseases; aging/geriatric diseases
storage temp.
−20°C
Gene Information
human ... PFKL(5211), PFKM(5213), PFKP(5214)
mouse ... PFKL(18641), PFKM(18642), PFKP(56421)
rat ... PFKL(25741), PFKM(65152), PFKP(60416)
Application
Phosphofructokinase (PFK) Activity Colorimetric Assay Kit has been used to measure the activity of phosphofructokinase.
Suitable for the measurement of phosphofructokinase activity, analysis of glucose metabolism and cell signaling and screening anti-cancer drugs in animal tissues (liver, brain, heart, muscles etc.) and cell culture (adherent or suspension cells).
Biochem/physiol Actions
The Phosphofructokinase Colorimetric Assay kit provides a simple and direct procedure for measuring PFK activity in a variety of samples. PFK activity is determined by a coupled enzyme assay, in which fructose-6-phosphate and ATP is converted to fructose-1,6-diphosphate and ADP by PFK. The ADP is converted by the enzyme mix to AMP and NADH. The resulting NADH reduces a colorless probe resulting in a colorimetric (450 nm) product proportional to the PFK activity present. One unit of PFK is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 7.4 at 37 °C.
General description
Phosphofructokinase (PFK) catalyzes the third step of glycolysis, the conversion of fructose-6-phosphate to fructose-1,6-diphosphate, the rate limiting step of glycolysis. PFK activity is highly regulated by multiple cofactors in addition to post-translational modifications and can be used to measure glycolytic flux in tissues. Deficiencies in PFK activity can result in the glycogen storage disease, glycogenosis type VII (Tarui′s disease), which is a glycogen storage disease.
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Danger
hcodes
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Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
Regulatory Information
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Abbriano R, et al.
Algal research, 32, 250-258 (2018)
Kai Zhou et al.
Cell death & disease, 9(10), 988-988 (2018-09-27)
Plastic phenotype convention between glioma stem cells (GSCs) and non-stem tumor cells (NSTCs) significantly fuels glioblastoma heterogeneity that causes therapeutic failure. Recent progressions indicate that glucose metabolic reprogramming could drive cell fates. However, the metabolic pattern of GSCs and NSTCs
T D Howard et al.
Genomics, 34(1), 122-127 (1996-05-15)
Phosphofructokinase (PFK) is a key rate-limiting enzyme in glycolysis and represents a major control point in the metabolism of glucose. There are at least three known isoforms of PFK in humans, referred to as the muscle, platelet, and liver forms
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