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MAK075

Sigma-Aldrich

Ascorbic Acid Assay Kit II

sufficient for 100 colorimetric tests

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Synonym(s):
FRASC Assay, Ferric Reducing/Antioxidant and Ascorbic Acid
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

General description

The new Ascorbic Acid Assay Kit, MAK505, is now available! Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.

Application

Ascorbic Acid Assay Kit II has been used to determine ascorbic acid concentration in vitreous sample from the eye.

Suitability

Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.

Principle

In this assay, ascorbic acid concentration is determined using the Ferric Reducing/Antioxidant and Ascorbic Acid (FRASC) assay. Fe3+ is reduced to Fe2+ by antioxidants present in the sample, which results in a colorimetric (593 nm) product. The addition of ascorbate oxidase to parallel samples oxidizes any ascorbic acid present allowing for the measurement of the ascorbic acid concentration.

replaced by

Product No.
Description
Pricing

WGK

WGK 3

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can product MAK075, Ascorbic Acid Assay kit, be used with tissue samples which have been frozen overnight?

    As long as the tissue is frozen in liquid nitrogen and stored at -80 °C, it should be fine. Since ascorbate is easiliy vulnerable to oxidation,  there could be some differences between the assayed values in fresh and frozen samples. 

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Karthik Murali et al.
PloS one, 11(3), e0149961-e0149961 (2016-03-02)
We investigated the spatial variation of vitreous oxygen consumption in enucleated porcine eyes. A custom made oxygen source was fabricated that could be localized to either the mid or posterior vitreous cavity and steady state vitreous oxygen tension was measured
Li-Zhen Zheng et al.
Biomaterials, 238, 119828-119828 (2020-02-12)
Magnesium (Mg)-based biometal attracts clinical applications due to its biodegradability and beneficial biological effects on tissue regeneration, especially in orthopaedics, yet the underlying anabolic mechanisms in relevant clinical disorders are lacking. The present study investigated the effect of magnesium (Mg)
Katarzyna Jezierska-Wozniak et al.
Journal of clinical medicine, 9(3) (2020-03-07)
Neurological disorders, including minimally conscious state (MCS), may be associated with the presence of high concentrations of reactive oxygen species within the central nervous system. Regarding the documented role of mesenchymal stem cells (MSCs) in oxidative stress neutralization, the aim

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