MAK056
Choline/Acetylcholine Quantification Kit
sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)
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Choline/Acetylcholine Assay Kit
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usage
sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)
detection method
colorimetric
fluorometric
storage temp.
−20°C
General description
Choline is an essential nutrient, commonly grouped with the B complex vitamins, that plays key roles in many biological processes. Choline is a precursor for the synthesis of acetylcholine, a critical neurotransmitter in both the peripheral and central nervous systems.
Application
Choline/Acetylcholine Quantification Kit has been used to quantify the amounts of choline and acetylcholine in samples.
Suitability
Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.
Principle
In this assay, choline concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the choline present. Acetylcholine levels can be determined by adding acetylcholinesterase to the reaction, which hydrolyzes acetylcholine to choline and acetate.
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Description
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
WGK
WGK 3
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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