MAK049
Phosphatidylcholine Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
Phosphatidylcholine Test Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric
fluorometric
relevant disease(s)
neurological disorders; gastrointestinal diseases; cancer
storage temp.
−20°C
General description
Phosphatidylcholine (PC) accounts for more than 50% of the phospholipids composing mammalian plasma membranes and is particularly enriched in the extracellular leaflet. PC is synthesized in the liver and is required for lipoprotein assembly and secretion. PC is cleaved by phospholipase D, which hydrolyses the choline head, producing the signaling intermediate phosphatidic acid.
Application
Phosphatidylcholine Assay Kit has been used to determine the concentration of phosphatidylcholine in samples.
Suitability
Suitable for the measurement of phosphatidylcholine in a variety of biological samples
Principle
In this assay, PC concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the PC present.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
Regulatory Information
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Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1862(12), 1469-1480 (2017)
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Sang-gyu L, et al.
Nuclear Medicine and Biology, 43(12), 781-787 (2016)
Riboflavin attenuates myocardial injury via LSD1-mediated crosstalk between phospholipid metabolism and histone methylation in mice with experimental myocardial infarction.
Peng W, et al.
Journal of Molecular and Cellular Cardiology, 115, 115-129 (2018)
Michelle S Prew et al.
Nature communications, 13(1), 3669-3669 (2022-06-28)
Very long-chain acyl-CoA dehydrogenase (VLCAD) is an inner mitochondrial membrane enzyme that catalyzes the first and rate-limiting step of long-chain fatty acid oxidation. Point mutations in human VLCAD can produce an inborn error of metabolism called VLCAD deficiency that can
Amanda L Brown et al.
The Journal of nutrition, 150(4), 775-783 (2019-12-19)
Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related death in the world. Choline deficiency has been well studied in the context of liver disease; however, less is known about the effects of choline supplementation in HCC. The
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