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Merck
CN

MAK038

NADP/NADPH Quantitation Kit

sufficient for 100 colorimetric tests

Synonym(s):

NADP/NADPH Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.25
eCl@ss:
32161000
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Product Name

NADP/NADPH Quantitation Kit, sufficient for 100 colorimetric tests

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−70°C

Application

NADP/NADPH (nicotinamide adenine dinucleotide phosphate) Quantitation Kit has been used for quantification of NADP/NADPH. It has been used to quantify NAD(P)H in order to determine SHMT2 (serine hydroxymethyltransferase 2) specific enzyme activity.
Suitable for the detection of NADP, NADPH and their ratio in a variety of biological samples including cell and tissue lysates

Biochem/physiol Actions

The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides, NADP (nicotinamide adenine dinucleotide phosphate) and NADPH, and their ratio. There is no requirement to purify NADP/NADPH from samples. This assay is specific for NADP and NADPH and does not detect NAD or NADH. NADPt (NADP and NADPH) or NADPH are quantified in a colorimetric assay (450 nm).

General description

Nicotinamide adenine dinucleotide phosphate (NADP) is an enzymatic cofactor involved in many redox reactions where it cycles between the reduced (NADPH) and oxidized (NADP) forms. NADP is also involved in biosynthetic reactions such as lipid and nucleic acid synthesis where it functions as a reducing agent. The oxidative branch of the Pentose Phosphate Pathway (PPP) is the major source of NADPH produced in animal cells.

pictograms

Health hazardCorrosion

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Danger

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class

8A - Combustible corrosive hazardous materials

Regulatory Information

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Jennifer L Szczytkowski et al.
The European journal of neuroscience, 28(9), 1867-1876 (2008-11-01)
Heroin administration alters the induction of nitric oxide, a molecule known to play a critical role in immune function. Previous research has shown that these alterations can be conditioned to environmental stimuli that have been associated with drug administration. Little
Jade Mims et al.
Radiation research, 183(3), 291-304 (2015-03-05)
While radiation therapy is commonly used for treating cancer, radiation resistance can limit long-term control of the disease. In this study, we investigated the reprogramming of the energy metabolism in radiosensitive and radioresistant head and neck squamous cell carcinomas (HNSCC)
Inge Grondman et al.
Journal of leukocyte biology, 106(1), 11-25 (2019-06-07)
Secondary infections are a major complication of sepsis and associated with a compromised immune state, called sepsis-induced immunoparalysis. Molecular mechanisms causing immunoparalysis remain unclear; however, changes in cellular metabolism of leukocytes have been linked to immunoparalysis. We investigated the relation
Metabolic engineering of Escherichia coli: increase of NADH availability by overexpressing an NAD+-dependent formate dehydrogenase.
Berri'os-Rivera S J, et al.
Metabolic engineering, 4(3), 217-229 (2002)
Mechanisms of action of isoniazid.
Timmins G S and Vojo D
Molecular Microbiology, 62(5), 1220-1227 (2006)

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