MAK038
NADP/NADPH Quantitation Kit
sufficient for 100 colorimetric tests
Synonym(s):
NADP/NADPH Assay Kit
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.25
Recommended Products
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−70°C
General description
Nicotinamide adenine dinucleotide phosphate (NADP) is an enzymatic cofactor involved in many redox reactions where it cycles between the reduced (NADPH) and oxidized (NADP) forms. NADP is also involved in biosynthetic reactions such as lipid and nucleic acid synthesis where it functions as a reducing agent. The oxidative branch of the Pentose Phosphate Pathway (PPP) is the major source of NADPH produced in animal cells.
Application
NADP/NADPH (nicotinamide adenine dinucleotide phosphate) Quantitation Kit has been used for quantification of NADP/NADPH. It has been used to quantify NAD(P)H in order to determine SHMT2 (serine hydroxymethyltransferase 2) specific enzyme activity.
Suitability
Suitable for the detection of NADP, NADPH and their ratio in a variety of biological samples including cell and tissue lysates
Principle
The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides, NADP (nicotinamide adenine dinucleotide phosphate) and NADPH, and their ratio. There is no requirement to purify NADP/NADPH from samples. This assay is specific for NADP and NADPH and does not detect NAD or NADH. NADPt (NADP and NADPH) or NADPH are quantified in a colorimetric assay (450 nm).
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
Regulatory Information
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