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Merck
CN

MAK037

NAD/NADH Quantitation Kit

sufficient for 100 colorimetric tests

Synonym(s):

NAD/NADH Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000

Key Documents

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Product Name

NAD/NADH Quantitation Kit, sufficient for 100 colorimetric tests

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

cancer

storage temp.

−20°C

Application

NAD/NADH Quantitation Kit has been used to quantify NAD+/NADH influenced by SIRT1 (sirtuin 1) activity (associated with ischemia-reperfusion injury) in the presence of trimetazidine.
Suitable for the detection of NADH and NAD in animal tissues (liver, kidney etc.), cell culture (adherent or suspension cells), serum or urine.

Biochem/physiol Actions

The NAD/NADH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides, NAD (nicotinamide adenine dinucleotide) and NADH, and their ratio without the requirement to purify NAD/NADH from samples. This assay is specific for NAD and NADH, and does not detect NADP or NADPH. NADt (NAD and NADH) or NADH are quantified in a colorimetric assay (450 nm).

General description

Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP (adenosine diphosphate)-ribosylation reactions and as a substrate for sirtuins.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
8J09K03370
8A17K03370
8B09K03370
8G29K03370
8H24K03370

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Mammalian sirtuins?emerging roles in physiology, aging, and calorie restriction.
Haigis M C and Leonard P G
Genes & Development, 20(21), 2913-2921 (2006)
Frédérique Moyrand et al.
Eukaryotic cell, 7(12), 2069-2077 (2008-09-30)
The genome of the basidiomycete pathogenic yeast Cryptococcus neoformans carries two UDP-glucose epimerase genes (UGE1 and UGE2). UGE2 maps within a galactose cluster composed of a galactokinase homologue gene and a galactose-1-phosphate uridylyltransferase. This clustered organization of the GAL genes
Deepanwita Banerjee et al.
BMC systems biology, 11(1), 51-51 (2017-04-28)
The leading edge of the global problem of antibiotic resistance necessitates novel therapeutic strategies. This study develops a novel systems biology driven approach for killing antibiotic resistant pathogens using benign metabolites. Controlled laboratory evolutions established chloramphenicol and streptomycin resistant pathogens
Andrea M Lencina et al.
mBio, 9(4) (2018-07-05)
The opportunistic pathogen Streptococcus agalactiae is the major cause of meningitis and sepsis in a newborn's first week, as well as a considerable cause of pneumonia, urinary tract infections, and sepsis in immunocompromised adults. This pathogen respires aerobically if heme
Haiping Wang et al.
Nature immunology, 21(3), 298-308 (2020-02-19)
Depleting regulatory T cells (Treg cells) to counteract immunosuppressive features of the tumor microenvironment (TME) is an attractive strategy for cancer treatment; however, autoimmunity due to systemic impairment of their suppressive function limits its therapeutic potential. Elucidating approaches that specifically
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