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MAK034

Sigma-Aldrich

Coenzyme A (CoA) Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

functional group

phospholipid

relevant disease(s)

endocrinological disorders, diabetes; cancer

shipped in

wet ice

storage temp.

−20°C

General description

Coenzyme A (CoA) is an essential metabolic cofactor synthesized from cysteine, pantothenate, and ATP (adenosine triphosphate). CoA plays important roles in many metabolic pathways, including the Tricarboxylic Acid cycle, and the synthesis and oxidation of fatty acids. One of the main functions of CoA is the carrying and transfer of acyl groups. Acylated deriviates, for example Acetyl-CoA, are critical intermediates in many metabolic reactions, particularly fatty acid and carbohydrate metabolism. CoA levels can be altered during starvation, and in conditions such as cancer, diabetes, and alcoholism.

Application

Coenzyme A (CoA) Assay Kit has been used to measure CoA levels.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of Coenzyme A (CoA) in a variety of biological samples including cell and tissue lysates

Principle

The Coenzyme A Assay kit is an easy and convenient assay to measure the CoA level in a variety of biological samples. CoA concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the CoA present. Typical sensitivities of detection for this kit are 0.1-10 nmol of CoA.

replaced by

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H319,H334

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Regulatory Information

常规特殊物品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8J01K03670
6C23K03670
7F02K03670
6M15K03670
5K16K03670

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Continuous fatty acid oxidation and reduced fat storage in mice lacking acetyl-CoA carboxylase 2.
Abu-Elheiga L, et al.
Science, 291(5513), 2613-2616 (2001)
Brandon C Rosen et al.
Antimicrobial agents and chemotherapy, 61(2) (2016-11-18)
Pyrazinamide (PZA) is a first-line tuberculosis (TB) drug that has been in clinical use for 60 years yet still has an unresolved mechanism of action. Based upon the observation that the minimum concentration of PZA required to inhibit the growth
Acetyl-CoA and the regulation of metabolism: mechanisms and consequences.
Current Opinion in Cell Biology, 33, 125-131 (2015)
Long-Chain Fatty Acyl-CoA Ligase FadD2 Mediates Intrinsic Pyrazinamide Resistance in Mycobacterium tuberculosis.
Rosen B C, et al.
Antimicrobial Agents and Chemotherapy, AAC-02130 (2016)
Pooja Gopal et al.
ACS infectious diseases, 2(9), 616-626 (2016-10-21)
Pyrazinamide (PZA) is a critical component of first- and second-line treatments of tuberculosis (TB), yet its mechanism of action largely remains an enigma. We carried out a genetic screen to isolate Mycobacterium bovis BCG mutants resistant to pyrazinoic acid (POA)
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