MAK033
ADP Colorimetric/Fluorometric Assay Kit
sufficient for 100 colorimetric or fluorometric tests
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric
fluorometric
storage temp.
−20°C
General description
The new ADP Assay Kit, MAK518, is now available! Adenosine diphosphate (ADP) is a nucleoside diphosphate that plays a critical role in energy transfer reactions. ADP is produced from adenosine triphosphate (ATP) via the action of ATPases. ADP also plays a critical role in platelet function. ADP, stored in platelet-dense granules, is released upon platelet activation where it acts on purinergic receptors to mediate intracellular signaling and platelet aggregation. ADP is a key agonist involved in thrombosis and hemostasis.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the measuerement of ADP in a variety of biological samples including cell and tissue lysates
Principle
In this assay, ADP (adenosine diphosphate) concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the ADP present.
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
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Molecular identification and characterization of the platelet ADP receptor targeted by thienopyridine antithrombotic drugs.
Foster C J, et al.
The Journal of Clinical Investigation, 107(12), 1591-1598 (2001)
ADP is not an agonist at P2X1 receptors: evidence for separate receptors stimulated by ATP and ADP on human platelets.
Mahaut-Smith M P, et al.
British Journal of Pharmacology, 131(1), 108-114 (2000)
Multinuclear magnetic resonance spectroscopy studies of brain purines in major depression.
Renshaw P F, et al.
The American Journal of Psychiatry, 158(12), 2048-2055 (2001)
Ana Luisa Palacios-Acedo et al.
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Platelet function can be modified by cancer cells to support tumor growth, causing alterations in the delicate hemostatic equilibrium. Cancer-cell and platelet interactions are one of the main pillars of Trousseau's syndrome: a paraneoplastic syndrome with recurring and migrating episodes
Jianmin Zhang et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 39(9), 1836-1848 (2018-04-17)
Neuronal preconditioning in vitro or in vivo with a stressful but non-lethal stimulus leads to new protein expression that mediates a profound neuroprotection against glutamate excitotoxicity and experimental stroke. The proteins that mediate neuroprotection are relatively unknown and under discovery.
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