MAK031
Phosphate Fluorometric Kit
sufficient for 100 fluorometric tests
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UNSPSC Code:
12352200
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 fluorometric tests
detection method
fluorometric
storage temp.
−20°C
General description
Phosphate is an essential component in living organisms and contributes to a variety of biological functions, including structural roles within nucleic acids, cellular membranes and bone. Phosphate is also important in the transport of cellular energy, nucleic acid metabolism and signal transduction. Hyperphosphatemia, a condition of excess phosphate levels in the blood, can lead to calcification of organs and interference with usage of other inorganic ions, such as iron, calcium, magnesium and zinc.
Suitability
Suitable for the detection of inorganic phosphate (Pi) produced through reactions involving ATPases, GTPases, 5′-nucleotidase, protein phosphatases, acidand alkaline phosphatases, phosphorylase, etc. from a variety of samples
Principle
The Phosphate Fluorometric Assay Kit provides a simple and direct procedure for measuring phosphate in a variety of samples. Phosphate reacts with maltose to produce glucose in the presence of an enzyme. The glucose is subsequently oxidized and reacts with a probe, resulting in a fluorometric (λex = 535/λem = 587 nm) product proportional to the amount of phosphate present. This kit has a linear range of detection between 50-250 pmoles.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 3
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Fluid, Electrolyte and Acid-Base Disorders (2014)
Advanced Biology for you. (2000)
Serum phosphate levels and mortality risk among people with chronic kidney disease.
Kestenbaum B, et al.
Journal of the American Society of Nephrology, 16(2), 520-528 (2005)
Biochemical, Physiological, and Molecular Aspects of Human Nutrition-E-Book (2013)
Sara D Siegel et al.
mBio, 10(1) (2019-02-21)
The widely conserved LytR-CpsA-Psr (LCP) family of enzymes in Gram-positive bacteria is known to attach glycopolymers, including wall teichoic acid, to the cell envelope. However, it is undetermined if these enzymes are capable of catalyzing glycan attachment to surface proteins.
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