Phosphate Fluorometric Kit

Phosphate is an essential component in living organisms and contributes to a variety of biological functions, including structural roles within nucleic acids, cellular membranes and bone. Phosphate is also important in the transport of cellular energy, nucleic acid metabolism and signal transduction. Hyperphosphatemia, a condition of excess phosphate levels in the blood, can lead to calcification of organs and interference with usage of other inorganic ions, such as iron, calcium, magnesium and zinc.

Suitable for the detection of inorganic phosphate (Pi) produced through reactions involving ATPases, GTPases, 5′-nucleotidase, protein phosphatases, acidand alkaline phosphatases, phosphorylase, etc. from a variety of samples

The Phosphate Fluorometric Assay Kit provides a simple and direct procedure for measuring phosphate in a variety of samples. Phosphate reacts with maltose to produce glucose in the presence of an enzyme. The glucose is subsequently oxidized and reacts with a probe, resulting in a fluorometric (λ_ex = 535/λ_em = 587 nm) product proportional to the amount of phosphate present. This kit has a linear range of detection between 50-250 pmoles.