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MAK025

Sigma-Aldrich

Iron Assay Kit

sufficient for 100 colorimetric tests

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UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric

relevant disease(s)

cancer; hematological disorder

storage temp.

−20°C

General description

Iron is a mineral that plays an essential role in many biological processes, including DNA synthesis, electron transport and oxygen transport. Iron is a transition element that can form a range of oxidation states, the most common being iron II (Fe2+ or ferrous iron) and iron III (Fe3+ or ferric iron). Iron-containing proteins participate in many reactions, often utilizing transitory changes in the oxidation state of iron to carry out chemical reactions.

Application

Iron assay kit has been used to determine the concentration of Fe2+ in wild type (WT) and Δ ferric uptake regulator (Fur) strains of A. baumannii.

Suitability

Suitable for the measurement of iron concentration in various samples

Principle

In this assay, iron is released by the addition of an acidic buffer. Samples may be tested directly to measure Fe2+ or reduced to measure total iron (Fe2+ and Fe3+). Released iron is reacted with a chromagen resulting in a colorimetric (593 nm) product, proportional to the iron present. The Iron Assay Kit provides a simple convenient means of measuring iron in a variety of biological samples.

Preparation Note

Kit is compatible with serum and tissue samples but not plasma samples.

related product

Product No.
Description
Pricing

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

WGK

WGK 3


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Manman Xu et al.
Journal of cellular physiology, 234(4), 4232-4242 (2018-08-23)
Previous studies have demonstrated an effect of estrogen on iron metabolism in peripheral tissues. The role of estrogen on brain iron metabolism is currently unknown. In this study, we investigated the effect and mechanism of estrogen on iron transport proteins.
Contributions of ferric uptake regulator Fur to the sensitivity and oxidative response of Acinetobacter baumannii to antibiotics
Ajiboye T o, et al.
Microbial Pathogenesis, 119, 35-41 (2018)
Ems T, Huecker MR
Biochemistry, Iron Absorption (2018)
Tippapha Pisithkul et al.
mBio, 10(3) (2019-05-23)
Biofilms are structured communities of tightly associated cells that constitute the predominant state of bacterial growth in natural and human-made environments. Although the core genetic circuitry that controls biofilm formation in model bacteria such as Bacillus subtilis has been well
Bin Lan et al.
Journal of integrative medicine, 18(4), 344-350 (2020-02-29)
Our previous research showed that Naotaifang (a compound traditional Chinese herbal medicine) extract (NTE) has clinically beneficial effects on neurological improvement of patients with acute cerebral ischemia. In this study, we investigated whether NTE protected acute brain injury in rats

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