Skip to Content
Merck
CN
All Photos(2)

Documents

MAK022

Sigma-Aldrich

Calcium Colorimetric Assay

sufficient for 250 colorimetric tests

Sign Into View Organizational & Contract Pricing

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 250 colorimetric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric

relevant disease(s)

neurological disorders; hematological disorder

storage temp.

2-8°C

General description

Calcium, the most abundant mineral in the human body, is a crucial intracellular element that is responsible for regulating many physiological and pathological processes. Calcium is found in either the free ion form or in bound complexes, for example the calcium phosphate and calcium carbonate complexes that make up bone tissue. Numerous physiological processes, including muscle contraction, cell adhesion, hormones/ neurotransmitters release, glycogen metabolism, cell proliferation/differentiation, blood clotting, nerve or synapthetic impulse transmission, and structural support of the skeleton are regulated by calcium signaling. Defects in the integrity of cell-specific calcium signaling systems may be associated with certain human diseases.

Application

Calcium Colorimetric Assay kit has been used to measure calcium concentration in hippocampal samples and MC3T3-E1 mouse osteoblast cell line, which were cultured in osteogenic induction medium.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for use with cell and tissue culture supernatants, urine, plasma, serum, fecal material, media, and other biological fluids.

Principle

In this assay, the calcium ion concentration is determined by the chromogenic complex formed between calcium ions and o-cresolphthalein, which is measured at 575 nm and is proportional to the concentration of calcium ions present. The linear range of detection for this kit is between 0.4-2.0 μg.

replaced by

Product No.
Description
Pricing

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Jing Che et al.
Life sciences, 182, 10-21 (2017-06-12)
The study aims to investigate the effects of Periostin gene silencing on tumor microenvironment and bone metastasis via the integrin-signaling pathway in lung cancer (LC). LC patients were divided into bone metastasis and non-bone metastasis groups; Healthy volunteers were selected
Santiago Pech-Pool et al.
International journal of molecular sciences, 21(4) (2020-02-26)
It is known that growth hormone (GH) is expressed in immune cells, where it exerts immunomodulatory effects. However, the mechanisms of expression and release of GH in the immune system remain unclear. We analyzed the effect of growth hormone-releasing hormone
Creatine?facilitated protection of stress caused by disrupted circadian rhythm
George B, et al.
Biological Rhythm Research, 49(1), 61- 75 (2018)
Kang Liu et al.
International journal of molecular medicine, 45(6), 1793-1802 (2020-04-03)
Calbindin‑D28K (Calb1) may protect human lens epithelial cells (HLECs) from apoptosis, which is a process resulting in individual cell death. The protective effects of Calb1 may be attributed to buffering high concentrations of Ca2+. The present study investigated the mechanisms
Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3-E1 cells
Wei J, et al.
Molecular Medicine Reports, 7391-7397 (2017)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service