MAK022
Calcium Colorimetric Assay
sufficient for 250 colorimetric tests
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Product Name
Calcium Colorimetric Assay, sufficient for 250 colorimetric tests
usage
sufficient for 250 colorimetric tests
application(s)
cosmetics
food and beverages
detection method
colorimetric
relevant disease(s)
neurological disorders; hematological disorder
storage temp.
2-8°C
Application
Calcium Colorimetric Assay kit has been used to measure calcium concentration in hippocampal samples and MC3T3-E1 mouse osteoblast cell line, which were cultured in osteogenic induction medium.
Suitable for use with cell and tissue culture supernatants, urine, plasma, serum, fecal material, media, and other biological fluids.
Biochem/physiol Actions
In this assay, the calcium ion concentration is determined by the chromogenic complex formed between calcium ions and o-cresolphthalein, which is measured at 575 nm and is proportional to the concentration of calcium ions present. The linear range of detection for this kit is between 0.4-2.0 μg.
Features and Benefits
Compatible with high-throughput handling systems.
General description
Calcium, the most abundant mineral in the human body, is a crucial intracellular element that is responsible for regulating many physiological and pathological processes. Calcium is found in either the free ion form or in bound complexes, for example the calcium phosphate and calcium carbonate complexes that make up bone tissue. Numerous physiological processes, including muscle contraction, cell adhesion, hormones/ neurotransmitters release, glycogen metabolism, cell proliferation/differentiation, blood clotting, nerve or synapthetic impulse transmission, and structural support of the skeleton are regulated by calcium signaling. Defects in the integrity of cell-specific calcium signaling systems may be associated with certain human diseases.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Creatine?facilitated protection of stress caused by disrupted circadian rhythm
George B, et al.
Biological Rhythm Research, 49(1), 61- 75 (2018)
Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3-E1 cells
Wei J, et al.
Molecular Medicine Reports, 7391-7397 (2017)
Wei Ji et al.
Molecular medicine reports, 16(5), 7391-7397 (2017-09-26)
Ankylosing spondylitis (AS) is characterized by the formation of bony spurs. Treatment of the resulting ankylosis, excessive bone formation and associated functional impairment, remain the primary therapeutic aims in research regarding this condition. Triptolide is the primary active component of
Kang Liu et al.
International journal of molecular medicine, 45(6), 1793-1802 (2020-04-03)
Calbindin‑D28K (Calb1) may protect human lens epithelial cells (HLECs) from apoptosis, which is a process resulting in individual cell death. The protective effects of Calb1 may be attributed to buffering high concentrations of Ca2+. The present study investigated the mechanisms
Calcium intake, calcium homeostasis and health.
Fan P, et al.
Food science and human wellness, 5(1), 8-16 (2016)
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Instructions
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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