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MAK005

Sigma-Aldrich

Phenylalanine Assay Kit

sufficient for 100 fluorometric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

relevant disease(s)

neonatal diseases; neurological disorders; pediatric diseases

storage temp.

−20°C

General description

Phenylalanine is a non-polar essential amino acid. In the liver, phenylalanine is converted to tyrosine, which is a precursor for multiple compounds including L-DOPA and melanin. Defects in the activity of phenylalanine hydroxylase result in the inherited metabolic disorder phenylketonuria (PKU). PKU results in the build up of phenylalanine and phenylalanine metabolites, and can result in growth defects and mental retardation if not treated.

Suitability

Suitable for L-phenylalanine detection in cell and tissue culture supernatants, serum, and other biological samples

Principle

Phenylalanine concentration is determined by a coupled enzyme assay, which results in the deamination of phenylalanine and the production of NADH which reacts with the probe resulting in a fluorescent (λex = 535 nm/λem = 587 nm) product, proportional to the phenylalanine present.

replaced by

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

No data available

Flash Point(C)

No data available

Regulatory Information

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Certificates of Analysis (COA)

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Phenylketonuria: an inborn error of phenylalanine metabolism.
Williams R A, et al.
The Clinical Biochemist. Reviews / Australian Association of Clinical Biochemists, 29(1), 31-31 (2008)
Nathan Crook et al.
ACS synthetic biology, 9(5), 1010-1021 (2020-04-24)
The development of robust engineered probiotic therapies demands accurate knowledge of genetic construct expression in the gut. However, the monetary and ethical costs of testing engineered strains in vertebrate hosts are incompatible with current high-throughput design-build-test cycles. To enable parallel
AMINO ACIDS | Metabolism
Encyclopedia of Food Sciences and Nutrition (Second Edition) (2003)
CTI Reviews
Clinical Chemistry, Principles, Procedures, Correlations (2016)
Protein and Amino Acid Metabolism
Essentials of Medical Biochemistry null
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Protocols

Enzymatic Method for Determining Phenylalanine (Phenylalanine Assay)

Assay protocol for the fluorometric detection of Phenylalanine in biological samples using the phenylalanine assay kit.

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