M9309
McCoy′s 5A Medium
With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
5A basal medium
Product Name
McCoy′s 5A Medium, Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: 2.2 g/L
L-glutamine: 0.21 g/L
phenol red: 0.011 g/L
glucose: 3.0 g/L (Dextro)
shipped in
ambient
storage temp.
2-8°C
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General description
The amino acid requirements for in vitro cultivation of Novikoff hepatoma cells were reported by McCoy and colleagues. These experiments were carried out using Basal Medium 5A, which was later modified to create McCoy′s 5A medium.
Application
McCoy′s 5A Medium has been used:
- to culture HCT-116?cells
- to culture clear cell renal cell carcinoma (ccRCC) cells Caki-1
- to maintain and culture human bone osteosarcoma Saos-2 cell line for in vitro cell studies
Biochem/physiol Actions
McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
动植物来源培养基
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