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M8537

Sigma-Aldrich

MCDB 131 Medium

With ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

131 Medium, Medium 131, Molecular, Cellular, and Development Biology 131 medium

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100 ML
CN¥458.16
500 ML
CN¥368.78
1 L
CN¥944.34
4 L
CN¥3,305.18

CN¥458.16

Available to ship onApril 17, 2025Details

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100 ML
CN¥458.16
500 ML
CN¥368.78
1 L
CN¥944.34
4 L
CN¥3,305.18

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

CN¥458.16

Available to ship onApril 17, 2025Details

Request a Bulk Order

Product Name

MCDB 131 Medium, With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

concentration

11.6 g/L

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

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Show Differences

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This Item
G901249767G6279
甘油 for molecular biology, ≥99.0%

G5516

甘油

甘油 ≥99.5%

G9012

甘油

甘油 BioUltra, anhydrous, ≥99.5% (GC)

49767

甘油

甘油 BioXtra, ≥99% (GC)

G6279

甘油

application(s)

life science and biopharma
microbiology
sample preservation

application(s)

lipidomics
liquid formulation
sample preservation

application(s)

-

application(s)

-

technique(s)

MALDI-MS: suitable, cryopreservation: suitable

technique(s)

immunofluorescence: suitable

technique(s)

-

technique(s)

-

grade

for molecular biology, reagent

grade

-

grade

for molecular biology

grade

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

solubility

water: 1 mL/mL, clear, colorless

solubility

water: 1 mL/mL, clear, colorless to faintly yellow

solubility

H2O: 5 M at 20 °C, clear, colorless

solubility

-

General description

MCDB-131 medium provides a defined and optimally balanced nutritional environment that selectively promotes the growth of specific cell types. MCDB-131 is designed to support clonal growth of human microvascular endothelial cells, including human umbilical vein endothelial cells, when supplemented with 0.7% FBSP, 10 ng/ml epidermal growth factor, and 1 μg/ml hydrocortisone. MCDB media is made for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP). 

Application

MCDB-131 Medium has been used:

  • as a component of the basal medium to culture bovine retinal endothelial cells[1]
  • to culture bovine lung microvascular endothelial cells[2]
  • for the isolation of porcine endocranial endothelial cells and porcine aortic endothelial cells[3]

Quantity

Formulated to contain 11.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.18 g/L sodium bicarbonate.

also commonly purchased with this product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000338761
    0000338757
    0000338759
    0000338438
    0000338438

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    Abdul Jaleel et al.
    Molecular and cellular biochemistry, 451(1-2), 1-10 (2018-06-24)
    Endocardial endothelium, which lines the chambers of the heart, is distinct in its origin, structure, and function. Characterization studies using genomics and proteomics have reported molecular signatures supporting the structural and functional heterogeneity of various endothelial cells. However, though functionally
    Kathleen M Job et al.
    Anesthesia and analgesia, 123(2), 382-393 (2016-06-23)
    The endothelial glycocalyx is an important component of the vascular permeability barrier, forming a scaffold that allows serum proteins to create a gel-like layer on the endothelial surface and transmitting mechanosensing and mechanotransduction information that influences permeability. During acute inflammation
    Thomas P Burke et al.
    eLife, 10 (2021-08-24)
    Arthropod-borne rickettsial pathogens cause mild and severe human disease worldwide. The tick-borne pathogen Rickettsia parkeri elicits skin lesions (eschars) and disseminated disease in humans; however, inbred mice are generally resistant to infection. We report that intradermal infection of mice lacking
    G S Ajithkumar et al.
    Cardiovascular toxicology, 16(4), 390-405 (2015-12-15)
    Endocardial endothelial cells (EECs), when compared with endothelial cells of arteries and veins, possess higher resistance to apoptosis-inducing anticancer agents. The mechanism of this resistance property is unknown. We have investigated the molecular mechanism, which contributes to increased cell survival
    Sandra S Hammer et al.
    Diabetologia, 64(7), 1674-1689 (2021-03-27)
    Homo sapiens evolved under conditions of intermittent food availability and prolonged fasting between meals. Periods of fasting are important for recovery from meal-induced oxidative and metabolic stress, and tissue repair. Constant high energy-density food availability in present-day society contributes to
    View All Related Papers

    Protocols

    Powdered Media Preparation Instructions

    Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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