M8407
Methotrexate hydrate
powder, suitable for cell culture, BioReagent
Synonym(s):
4-Amino-10-methylfolic acid hydrate, L-4-Amino-N10-methylpteroylglutamic acid hydrate, L-Amethopterin hydrate, Antifolan hydrate, MTX hydrate, Methylaminopterin hydrate
About This Item
Recommended Products
Product Name
Methotrexate hydrate, powder, BioReagent, suitable for cell culture, ≥98% (HPLC)
product line
BioReagent
Quality Level
Assay
≥98% (HPLC)
form
powder
technique(s)
cell culture | mammalian: suitable
solubility
H2O: insoluble
antibiotic activity spectrum
neoplastics
parasites
Mode of action
enzyme | inhibits
storage temp.
−20°C
SMILES string
[H]O[H].CN(Cc1cnc2nc(N)nc(N)c2n1)c3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O
InChI
1S/C20H22N8O5.H2O/c1-28(9-11-8-23-17-15(24-11)16(21)26-20(22)27-17)12-4-2-10(3-5-12)18(31)25-13(19(32)33)6-7-14(29)30;/h2-5,8,13H,6-7,9H2,1H3,(H,25,31)(H,29,30)(H,32,33)(H4,21,22,23,26,27);1H2/t13-;/m0./s1
InChI key
FPJYMUQSRFJSEW-ZOWNYOTGSA-N
Gene Information
human ... DHFR(1719)
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Related Categories
Application
- as a dihydrofolate (DHFR) inhibitor in sphere forming assay culture
- as a component of dulbecco′ modified Eagle′s medium (DMEM) for selection of transfected cells
- in viability and co-culture assays
Biochem/physiol Actions
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Oral - Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I make a solution of Methotrexate hydrate, Product M8407?
Methoxtrexate is soluble in 1M NaOH or NH4OH (10 mg/ml). For use in cell culture, sodium carbonate buffer (pH 9.0-9.5) can be used to dissolve methotrexate.
How do I store a stock solution of Methotrexate hydrate, Product M8407?
A sterile solution of methotrexate can be stored at 2-8 °C for approximately 30 days.
How do I sterilize a solution of Methotrexate hydrate, Product M8407?
A solution of methotrexate can be sterile filtered using a 0.22 mm filter
How often does Methotrexate hydrate, Product M8407, need to be added to the media during selection?
Fresh methotrexate should be added each time the culture media is changed. Regardless of cell growth, most media will need to be changed at least once a week.
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