M8316
Anti-MRP2 antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-ABCC2, Anti-Multidrug Resistance Associated Protein 2, Anti-cMOAT, Anti-cMRP
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About This Item
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~190 kDa
species reactivity
rat, human
technique(s)
indirect immunofluorescence: 1:100 using paraformaldehyde-fixed HepG2 cells
indirect immunofluorescence: 1:100 using rat liver frozen sections
western blot: 1:1,000 using whole extract of HepG2 human hepatoblastoma cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ABCC2(1244)
rat ... Abcc2(25303)
Related Categories
General description
Multidrug Resistance-associated Protein 2 (MRP2), also called as canalicular Multispecific Organic Anion Transporter (cMOAT), is a member of the CFTR/MRP (ABCC) subfamily of the large ATP-Binding Cassette (ABC) transporter family of transmembrane proteins. MRP2 is normally expressed in the liver, gallbladder, kidney proximal tubules, placenta, duodenum and small intestine. Localization pattern appears to vary in different species. MRP2 is predominantly localized to the apical membrane of polarized cells.
Multidrug resistance associated protein 2 (MRP2) is expressed in endothelial cells of the blood-brain barrier, various carcinoma cell lines and tumors. The gene encoding MRP2 is localized on human chromosome 10q24 and consists of 32 exons.
Specificity
Additional lower bands including an approx. 175 kDa band representing an immature unglycosylated form may be detected in various extract preparations.
Immunogen
synthetic C-terminal peptide corresponding to amino acids 1528-1545 of human MRP2 conjugated to KLH.
Application
Anti-MRP2 antibody produced in rabbit has been used in:
- immunoblotting
- western blotting
- immunofluorescence
Biochem/physiol Actions
Multidrug Resistance-associated Protein 2 (MRP2) transports endogenous and exogenous anionic conjugates from hepatocytes to the bile. Thus, it contributes to bile flow and plays a role in detoxification and defense against oxidative stress. Patients with the rare autosomal recessive Dubin-Johnson Syndrome develop a mild liver disease caused by MRP2 deficiency. Up regulation of MRP2 expression may be found in hepatocellular carcinomas.
Multidrug resistance associated protein 2 (MRP2) is a transporter which is involved in the efflux of various xenobiotic compounds like doxorubicin and methotrexate.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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A L Dzierlenga et al.
Drug metabolism and disposition: the biological fate of chemicals, 44(11), 1799-1807 (2016-09-09)
Interindividual variability in drug response in nonalcoholic steatohepatitis (NASH) can be mediated by altered regulation of drug metabolizing enzymes and transporters. Among these is the mislocalization of multidrug resistance-associated protein (MRP2)/Mrp2 away from the canalicular membrane, which results in decreased
Yan Liu et al.
PloS one, 9(1), e82681-e82681 (2014-01-10)
Methotrexate (MTX) is a key agent for the treatment of childhood acute lymphoblastic leukemia (ALL). Increased MTX plasma concentrations are associated with a higher risk of adverse drug effects. ATP-binding cassette subfamily C member 2 (ABCC2) is important for excretion
Nonalcoholic steatohepatitis modulates membrane protein retrieval and insertion processes
Dzierlenga AL, et al.
Drug Metabolism and Disposition, 44(11), 1799-1807 (2016)
Barbara Schaarschmidt et al.
Theranostics, 8(14), 3766-3780 (2018-08-08)
Rationale: The liver is a central organ not only for metabolism but also immune function. Life-threatening infections of both bacterial and fungal origin can affect liver function but it is yet unknown whether molecular changes differ depending on the pathogen.
Analysis of ATP-binding cassette transporter expression in drug-selected cell lines by a microarray dedicated to multidrug resistance
Annereau JP, et al.
Molecular Pharmacology, 66(6), 1397-1405 (2004)
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