M8195
Monoclonal Anti-phospho-MDMX (pTyr99) antibody produced in mouse
~2 mg/mL, clone PH-MDMX-169, purified immunoglobulin, buffered aqueous solution
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Synonym(s):
Anti-HDM4, Anti-HDMX, Anti-MDM4, Anti-MGC132766, Anti-MRP1
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PH-MDMX-169, monoclonal
form
buffered aqueous solution
mol wt
antigen ~80 kDa
species reactivity
human
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
technique(s)
indirect ELISA: suitable
indirect immunofluorescence: suitable
western blot: 1-2 μg/mL using total cell extract of HEK-293T cells co-transfected with human MDMX and a specific kinase
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr99)
Gene Information
human ... MDM4(4194)
General description
MDMX is a nuclear protein and the gene encoding it is localized on human chromosome 1q32.1.
Monoclonal Anti-phospho-MDMX (pTyr99) (mouse IgG2b isotype) is derived from the hybridoma PH-MDMX-169 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Mouse double minute 4 (MDM4) or MDMX comprises p53 binding domain, central acidic region, zinc and interesting new gene (RING) finger domain and is a phosphorylated protein. MDM4 gene is mapped to human chromosome 1q32.1.
Specificity
Monoclonal Anti-phospho-MDMX (pTyr99) recognizes human phosphorylated MDMX (pTyr99) (~ 80 kDa). The antibody does not recognize the non-phosphorylated MDMX, which is produced when MDMX is expressed with a kinase mutant.
Immunogen
synthetic phosphopeptide corresponding to amino acids 93-106 (pTyr99) of human MDMX.
Application
Monoclonal Anti-phospho-MDMX (pTyr99) antibody produced in mouse may be used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunofluorescence
- immunoblotting
Biochem/physiol Actions
MDMX associates with tumor suppressor p53 and inhibits its activity. It is upregulated in many tumors.
Studies began to unravel the regulation of murine double minute X (MDMX) in response to stress conditions, involving post-translational modifications and protein-protein interactions.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Qiong Yu et al.
Diagnostic pathology, 9, 71-71 (2014-03-29)
The p53 tumor suppressor gene is mutated or deleted in nearly half of human cancers. The murine double minute 2 (Mdm2) and Mdmx represent two important cellular regulators of p53. The aim of this study was to evaluate the abnormalities
F Mancini et al.
Current genomics, 10(1), 42-50 (2009-09-02)
MDM family proteins are crucial regulators of the oncosuppressor p53. Alterations of their gene status, mainly amplification events, have been frequently observed in human tumors.MDM4 is one of the two members of the MDM family. The human gene is located
Xiaowen Wang et al.
Brain research, 1537, 260-266 (2013-09-03)
The product of the MDMX (or MDM4) gene is structurally related to the MDM2 oncoprotein and is also capable of interacting with the tumor suppressor protein p53. The MDM4 gene is overexpressed in several human tumors, while its product can
Samanta Salvi et al.
International journal of molecular sciences, 15(7), 12458-12468 (2014-07-16)
Patients with non-muscle invasive bladder cancer (NMIBC) generally have a high risk of relapsing locally after primary tumor resection. The search for new predictive markers of local recurrence thus represents an important goal for the management of this disease. We
H Zhang et al.
Oncogene, 34(44), 5560-5569 (2015-02-24)
Inactivation of the retinoblastoma protein (RB) has a major role in the development of human malignancies. We have previously shown that MDM2, an ubiquitin E3 ligase and major negative regulator of p53, binds to and promotes proteasome-mediated degradation of RB.
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