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M8064

Sigma-Aldrich

Anti-Myosin IIA, non muscle antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-BDPLT6, Anti-DFNA17, Anti-EPSTS, Anti-FTNS, Anti-MATINS, Anti-MHA, Anti-NMHC-II-A, Anti-NMMHC-IIA, Anti-NMMHCA
MDL number:
MFCD03455610
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~200 kDa

species reactivity

rat, human, canine

packaging

antibody small pack of 25 μL

technique(s)

indirect immunofluorescence: 1:100 using cultured rat NRK cells
microarray: suitable
western blot: 1:1,000 using whole cell extracts of cultured dog MDCK kidney cells and cultured human Jurkat cells

UniProt accession no.

P35579

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYH9(4627)
rat ... Myh9(25745)

General description

Myosin heavy chain 9 (MYH9) gene codes for the nonmuscle myosin heavy chain IIA.
Myosin heavy chain 9 is expressed in platelets and the gene encoding it is localized on chromosome 22.

Immunogen

synthetic peptide corresponding to amino acids 1949-1960 of human nonmuscle myosin IIA.

Application

Anti-Myosin IIA, non muscle antibody produced in rabbit has been used in:
  • immunofluorescence
  • immunohistochemistry
  • fixed cell staining/ immunofluorescence staining
  • immunoblot
  • immunoprecipitation

Biochem/physiol Actions

Nonmuscle myosin II is involved in cell motility and adhesion, cytokinesis, vesicular transport, intracellular force generation and in morphogenesis during development. Its activity is regulated by light chain and possibly heavy chain phosphorylation and by association with proteins such as Mts1. Mutations in the NMHCA gene are found in several syndromes associated with megakaryocyte/platelet/leukocyte disorders. Mutations in the MYH9 gene causes a spectrum of macrothrombocytopenia disorders with neutrophil inclusions, termed as MYH9 disorders.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品
含少量动物源组分生物产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The role of stress fibers in the shape determination mechanism of fish keratocytes
Nakata T, et al.
Biophysical Journal, 110(2), 481-492 (2016)
Xiong Liu et al.
The Journal of biological chemistry, 291(48), 24828-24837 (2016-10-05)
Mammalian cells express three Class II nonmuscle myosins (NM): NM2A, NM2B, and NM2C. The three NM2s have well established essential roles in cell motility, adhesion, and cytokinesis and less well defined roles in vesicle transport and other processes that would
Nonmuscle myosin-2: mix and match
Heissler SM and Manstein DJ
Cellular and Molecular Life Sciences, 70(1), 1-21 (2013)
Y Chen et al.
Journal of thrombosis and haemostasis : JTH, 11(12), 2163-2175 (2013-10-30)
Mutations in the MYH9 gene cause autosomal dominant MYH9-related diseases (MYH9-RD) that associate macrothrombocytopenia with various other clinical conditions. The mechanisms giving rise to giant platelets remain poorly understood. To study the proplatelet formation (PPF) derived from megakaryocytes (MKs) generated
C Ling et al.
Genetics and molecular research : GMR, 14(1), 1008-1016 (2015-03-03)
Genetic factors play an important role in type 2 diabetes (T2D) complications. Alteration of cerebrovascular blood flow (CBF) is a direct result of cerebrovascular diseases. However, few studies have reported the role of genetics on CBF in patients with T2D.
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