M7939
Anti-Myosin IIB, non-muscle antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
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Synonym(s):
Anti-Myosin-10
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~200 kDa
species reactivity
rat, human, canine
technique(s)
western blot (chemiluminescent): 1:200 using whole cell extracts of cultured dog MDCK kidney cells, cultured rat NRK cells, and cultured human Jurkat cells.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
rat ... Myh10(79433)
Related Categories
General description
Myosin IIB (nonmuscle) is a relatively abundant, widespread two headed myosin composed of an N-terminal motor domain, a light chain binding neck region, a coiled-coil region, and a nonhelical C-terminal domain. It forms a heterohexamer composed of a pair of heavy chains and two pairs of light chains. Myosin IIB is expressed in embryonic cardiac myocytes.
Specificity
Anti-Myosin IIB, non-muscle antibody is specific for the heavy chain of human myosin IIB (nonmuscle). The antibody also reacts with myosin in dogs and rats.
Immunogen
Synthetic peptide corresponding to amino acid residues 1965-1976 of the heavy chain of human myosin IIB (nonmuscle) conjugated to KLH. This sequence is identical in rabbit and differs by one and two amino acids from the respective bovine and rat sequences.
Application
Anti-Myosin IIB, non-muscle antibody produced in rabbit has been used in:
- immunoblotting
- stimulated emission depletion (STED) nanoscopy
- immunofluorescence
Biochem/physiol Actions
Myosin IIB plays a key role in the COS-7 cell cytokinesis and is need for the initiation of ring constriction.
Myosins are actin-dependent motors that have 15 classes based on their phylogenetics . Myosin IIb regulates memory and synaptic plasticity functions. Nonmuscle myosin IIb has been implicated in fibroblast movement and erythroblast enucleation .
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
The role of pulmonary vascular contractile protein expression in pulmonary arterial hypertension
Konik EA, et al.
Journal of Molecular and Cellular Cardiology, 65(12), 147-155 (2013)
Myosin II recruitment during cytokinesis independent of centralspindlin-mediated phosphorylation
Beach JR and Egelhoff TT
Test, 284(40), 27377-27383 (2009)
Life without double-headed non-muscle myosin II motor proteins
Betapudi V
Frontiers in Chemistry, 2, 45-45 (2014)
Non-muscle myosin heavy chain IIA and IIB interact and co-localize in living cells: relevance for MYH9-related disease
Marini M, et al.
International Journal of Molecular Medicine, 17(5), 729-736 (2006)
Nonmuscle myosin II exerts tension but does not translocate actin in vertebrate cytokinesis
Ma X, et al.
Proceedings of the National Academy of Sciences of the USA, 109(12), 4509-4514 (2012)
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