M7931
Monoclonal Anti-Mcm7 antibody produced in mouse
clone DCS-141, ascites fluid
Synonym(s):
Anti-Cdc47
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
DCS-141, monoclonal
mol wt
antigen ~80 kDa
contains
15 mM sodium azide
species reactivity
human, Xenopus
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1:2,000 using HeLa cell nuclear preparation
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MCM7(4176)
General description
Minichromosome maintenance complex component 7 (MCM7) is part of the pre-replication complex.
Monoclonal Anti-MCM7 (CDC47) (mouse IgG1 isotype) is derived from the DCS-141 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The MCM7 gene is mapped to human chromosome 7q22.1.
Specificity
Recognizes human Mcm7 and also a weaker lower molecular weight doublet.
Immunogen
recombinant human Mcm7 (Cdc47) protein
Application
Monoclonal Anti-Mcm7 antibody produced in mouse has been used in:
- immunoblotting
- immunocytochemistry
- immunoprecipitation
Biochem/physiol Actions
MCM7 levels do not vary with cell cycle, but the expression and nascent synthesis of MCM7 occurs late in the cell cycle, coinciding with mitosis. MCM7 immunolocalization may be used as an index of cell proliferation in tissue sections. Monoclonal antibody reacting specifically with MCM7 is also a useful tool in the investigation of the molecular mechanisms behind DNA replication and its restriction to S phase. MCM7 may play a role in normal and neoplastic cell growth. MCM7 favors hepatocellular carcinoma progression.
Minichromosome maintenance complex component 7 (MCM7) has been shown to possess DNA helicase activity and is crucial for initiating replication.
Target description
Mcm7 (Cdc47) belongs to the minichromosome maintenance family.
Physical form
The product is provided as ascites fluid with 15 mM sodium azide (see MSDS) as a preservative.
Storage and Stability
For continuous use, store at 2-8 degree C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Kai Qu et al.
Cell death & disease, 8(2), e2603-e2603 (2017-02-10)
DNA replication is a central procedure of cell proliferation, whereas aberrant DNA replication is indicated to be a driving force of oncogenesis. Minichromosome maintenance complex component 7 (MCM7) plays an essential role in initiating DNA replication. To investigate the potential
Masatoshi Takagi et al.
The FEBS journal, 275(24), 6109-6122 (2008-11-20)
Sister chromatid cohesion is important for the correct alignment and segregation of chromosomes during cell division. Although the cohesin complex has been shown to play a physical role in holding sister chromatids together, its loading onto chromatin is not sufficient
Takatsugu Kan et al.
Gastroenterology, 136(5), 1689-1700 (2009-05-08)
Barrett's esophagus (BE) is a highly premalignant disease that predisposes to the development of esophageal adenocarcinoma (EAC); however, the involvement of microRNAs (miRs) in BE-EAC carcinogenic progression is not known. Esophageal cultured cells (HEEpiC, QhTRT, ChTRT, GihTRT, and OE-33) and
B K Tye
Annual review of biochemistry, 68, 649-686 (2000-06-29)
The MCM proteins are essential replication initiation factors originally identified as proteins required for minichromosome maintenance in Saccharomyces cerevisiae. The best known among them are a family of six structurally related proteins, MCM2-7, which are evolutionally conserved in all eukaryotes.
Katerina Vrzalikova et al.
Pathogens (Basel, Switzerland), 1(2), 83-101 (2012-01-01)
B-lymphocyte-induced maturation protein 1 (BLIMP1) exists as two major isoforms, α and β, which arise from alternate promoters. Inactivation of the full length BLIMP1α isoform is thought to contribute to B cell lymphomagenesis by blocking post-germinal centre (GC) B cell
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