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M7819

Sigma-Aldrich

β-Mannosidase from Helix pomatia

BioReagent, Proteomics Grade

Synonym(s):

β-D-Mannoside mannohydrolase

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About This Item

CAS Number:
9025-43-8
Enzyme Commission number:
3.2.1.25 (BRENDA, IUBMB)
MDL number:
MFCD00130638
UNSPSC Code:
12352204

biological source

Helix pomatia

product line

BioReagent

Assay

≥90% (SDS-PAGE)

form

lyophilized solid

quality

Proteomics Grade

mol wt

94 kDa

storage temp.

−20°C

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Packaging

Provided with 5× concentrate reaction buffer

Unit Definition

One unit will hydrolyze 1 μmole of p-nitrophenyl β-D-mannopyranoside to p-nitrophenol (measured at 400 nm) and D-mannose per minute at pH 4.0 at 37 °C.

Physical form

Enzyme is lyophilized from 10 mM sodium acetate buffer, pH 4.0, containing BSA and sodium chloride.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Jia Zheng et al.
Bioresource technology, 118, 257-264 (2012-06-19)
An industrial medium, Corn Steep Liquor Powder Dextrose (CSD medium) was developed for constitutive expression of recombinant β-mananase by Pichia pastoris. The β-mananase activity (513 U/mL) with CSD medium was 1.64- and 2.5-fold higher than with YPD and BSM in
Yuya Kumagai et al.
Biochimie, 94(12), 2783-2790 (2012-09-27)
Mannanase is an important enzyme involved in the degradation of mannan, production of bioactive oligosaccharides, and biobleaching of kraft pulp. Mannanase must be thermostable for use in industrial applications. In a previous study, we found that the thermal stability of
Guojie Jin et al.
Bioresource technology, 111, 378-382 (2012-03-01)
Lipids produced by oleaginous microorganisms are a potential feedstock for biodiesel production and chemical synthesis. Yet, the costs of microbial lipids remain high, partially because the lipid recovery process is tedious and costly. In the present study, enzyme-assisted extraction of
María del Carmen Rodríguez-Gacio et al.
Journal of experimental botany, 63(11), 3976-3988 (2012-05-04)
The softening and degradation of the cell wall (CW), often mannan enriched, is involved in several processes during development of higher plants, such as meristematic growth, fruit ripening, programmed cell death, and endosperm rupture upon germination. Mannans are also the
Thi Huynh et al.
BMC genomics, 12 Suppl 3, S22-S22 (2012-03-06)
Lysosomal β-D-mannosidase is a glycosyl hydrolase that breaks down the glycosidic bonds at the non-reducing end of N-linked glycoproteins. Hence, it is a crucial enzyme in polysaccharide degradation pathway. Mutations in the MANBA gene that codes for lysosomal β-mannosidase, result
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