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M7133

4-Methylumbelliferyl sulfate potassium salt

Name: 4-Methylumbelliferyl sulfate potassium salt
Brand: SIGMA

sulfatase substrate

Synonym(s):

Potassium 4-methylumbelliferyl sulfate

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About This Item

Empirical Formula (Hill Notation):

C₁₀H₇KO₆S

CAS Number:

15220-11-8

Molecular Weight:

294.32

Beilstein:

3803203

EC Number:

239-272-2

MDL number:

MFCD00016970

UNSPSC Code:

12352204

PubChem Substance ID:

24897141

NACRES:

NA.32

Quality Level

200

Assay

≥99% (HPLC)

form

powder

solubility

water: 5 mg/mL, clear, colorless

fluorescence

λ_ex 334 nm; λ_em 370 nm (pH 10.4)
λ_ex 360 nm; λ_em 449 nm (Reaction products)

storage temp.

−20°C

SMILES string

[K+].CC1=CC(=O)Oc2cc(OS([O-])(=O)=O)ccc12

InChI

1S/C10H8O6S.K/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14;/h2-5H,1H3,(H,12,13,14);/q;+1/p-1

InChI key

CSOCSPXOODWGLJ-UHFFFAOYSA-M

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General description

4-Methylumbelliferyl sulfate (4MUS), a general substrate, is a polar metabolite of 4-methylumbelliferone (4MU).

Application

4-Methylumbelliferyl sulfate potassium salt has been used as a substrate in coupled fluorescence sulfotransferase assay to regenerate 3′-phosphoadenosine 5′-phosphosulfate (PAPS) from 3′-phosphoadenosine 5′-phosphate for cerebroside sulfotransferase (CST) enzyme activity measurements. It has also been used as a substrate in arylsulfatase assay/4-methylumbelliferyl sulfate (4-MUS) assay to test the enzymatic activity of the protein-bead complex.

Biochem/physiol Actions

4-Methylumbelliferyl sulfate (4MUS) is capable of undergoing desulfation and involve in futile cycling with 4MU. It can be utilized to measure the arylsulfatase activity of several sulfatases.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Uptake of sulfate conjugates by isolated rat hepatocytes.

A M Hassen et al.

Drug metabolism and disposition: the biological fate of chemicals, 24(7), 792-798 (1996-07-01)

The uptake of estrone sulfate (E1S; 1 to 400 microM), harmol sulfate (HS; 5 to 900 microM), and 4-methylumbelliferyl sulfate (4MUS; 5 to 1000 microM) was investigated in isolated rat hepatocytes in the presence or absence of inhibitors. Uptake of

A fluorimetric assay of steroid sulphatase in leukocytes: evidence for two genetically different enzymes with arylsulphatase C activity.

O P van Diggelen et al.

Journal of inherited metabolic disease, 12(3), 273-280 (1989-01-01)

Arylsulphatase C (ASC) activity in leukocytes and fibroblasts measured with 4-methylumbelliferylsulphate, is caused by at least two genetically different sulphatases. One of these is steroid sulphatase (STS). Depending on the substrate concentration, about 10-50% of the ASC activity in leukocytes

High-performance liquid chromatographic method for the direct determination of 4-methylumbelliferone and its glucuronide and sulfate conjugates. Application to studies in the single-pass in situ perfused rat intestine-liver preparation.

C L Zimmerman et al.

Journal of chromatography, 563(1), 83-94 (1991-01-18)

A direct high-performance liquid chromatographic (HPLC) assay was developed for the separation and determination of 4-methylumbelliferone (4MU) and its glucuronide (MUG) and sulfate (MUS) conjugates in the cell-free perfusate ("plasma") from in situ perfused rat intestine-liver preparation. In addition, a

Regional expression and activity of breast cancer resistance protein (Bcrp/Abcg2) in mouse intestine: overlapping distribution with sulfotransferases.

Junichi Enokizono et al.

Drug metabolism and disposition: the biological fate of chemicals, 35(6), 922-928 (2007-03-14)

Breast cancer resistance protein (Bcrp/Abcg2) is a member of the ATP-binding cassette transporter family with the ability to transport a variety of sulfate conjugates. In the present study, the regional expression and activity of Bcrp and sulfotransferases (SULTs/Sults) were investigated

A high-throughput cell-based assay pipeline for the preclinical development of bacterial DsbA inhibitors as antivirulence therapeutics.

Anthony D Verderosa et al.

Scientific reports, 11(1), 1569-1569 (2021-01-17)

Antibiotics are failing fast, and the development pipeline remains alarmingly dry. New drug research and development is being urged by world health officials, with new antibacterials against multidrug-resistant Gram-negative pathogens as the highest priority. Antivirulence drugs, which inhibit bacterial pathogenicity

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