M7024
Murashige and Skoog Shoot Multiplication Medium A
powder, suitable for plant cell culture
Synonym(s):
MSMA
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.72
form
powder
Quality Level
technique(s)
cell culture | plant: suitable
application(s)
agriculture
shipped in
ambient
storage temp.
2-8°C
Application
Murashige and Skoog Shoot Multiplication Medium A is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).
Formula variant
With the macro- and micronutrients, vitamins, sucrose, IAA, sodium phosphate, adenine and 2iP as described by Huang and Murashige (1976) TCA Manual.
Media Formulation
Media Formulation
Preparation Note
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Shoot Multiplication Medium A (M7024 is a variant media) with macro- and micronutrients, vitamins, sucrose, IAA, sodium phosphate, adenine and 2iP.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Ox. Sol. 3
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
动植物来源培养基
Certificates of Analysis (COA)
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Plant tissue culture media: major constituents, their preparation and some applications.
Huang L, Murashige T.
Tissue Antigens, 3, 539-548 (1976)
R H Smith et al.
In vitro, 13(5), 329-334 (1977-05-01)
Defined in vitro conditions for callus initiation by Gossypium arboreum L. were determined, and different tissues were evaluated as explant sources, Environmental conditions tested included light versus dark, and low light versus high light. Different nutrient media as well as
Akasaka et al.
Plant science : an international journal of experimental plant biology, 156(2), 169-175 (2000-08-11)
Bud primordia were induced from leaf segments, which were harvested from young seedlings of Spanish type peanut (Arachis hypogaea L. cv. Chico), on 0.8% agar-solidified medium containing Murashige and Skoog (MS) basal salts supplemented with B5 vitamins, 1 mg/l NAA
Nor Azlina Hasbullah et al.
Pakistan journal of biological sciences : PJBS, 11(11), 1449-1454 (2008-09-27)
Regeneration potentials in Gerbera jamesonii Bolus ex. Hook f. from tissues culture system was studied using leaf, petiole and root explants. In vitro regeneration, callus induction and root formation were optimized by manipulation of growth regulators during organogenesis. Various kinds
Regulation of lycopene formation in cell suspension culture of VFNT tomato (Lycopersicon esculentum) by CPTA, growth regulators, sucrose, and temperature
Robertson GE, Mahoney NE
Journal of Experimental Botany, 46, 667-673 (1995)
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