M6313
Mimetic Green 1 Ligand Affinity Adsorbent
suspension
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Application
Ligand affinity adsorbents are used in protein chromatography, affinity chromatography and dye resins. Ligand affinity adsorbents have been used to describe a simple protocol for the purification of Taq Pol from E. coli lysates as well as for the purification of other DNA polymerases.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
WGK
WGK 1
Flash Point(F)
95.0 °F
Flash Point(C)
35 °C
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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B V Thage et al.
Journal of applied microbiology, 96(3), 593-602 (2004-02-14)
Purification and characterization of an aminotransferase (AT) specific for the degradation of branched-chain amino acids from Lactobacillus paracasei subsp. paracasei CHCC 2115. The purification protocol consisted of anion exchange chromatography, affinity chromatography and hydrophobic interaction chromatography. The enzyme was found
S C Woodcock et al.
The Biochemical journal, 313 ( Pt 2), 415-421 (1996-01-15)
CysG, also known as uroporphyrinogen III methylase and sirohaem synthase (CysG; EC 2.1.1.107), is a multifunctional enzyme that is able to transform uroporphyrinogen III into sirohaem via two S-adenosyl-L-methionine (AdoMet)-dependent transmethylations, an NAD(+)-dependent dehydrogenation and a ferrochelation. The apparent tight
Michael Kotik et al.
Biochimica et biophysica acta, 1760(2), 245-252 (2005-12-14)
Purification of a novel enantioselective epoxide hydrolase from Aspergillus niger M200 has been achieved using ammonium sulphate precipitation, ionic exchange, hydrophobic interaction, and size-exclusion chromatography, in conjunction with two additional chromatographic steps employing hydroxylapatite, and Mimetic Green. The enzyme was
J Ni et al.
The Journal of biological chemistry, 272(16), 10853-10858 (1997-04-18)
A new member of the human cystatin superfamily, called cystatin E, has been found by expressed sequence tag (EST) sequencing in amniotic cell and fetal skin epithelial cell cDNA libraries. The sequence of a full-length amniotic cell cDNA clone contained
Jeannie Horak et al.
Analytical and bioanalytical chemistry, 400(8), 2349-2359 (2010-12-22)
The new affinity-type Mimetic Ligand™ B14 was coupled with a 1,2-diaminoethane spacer (2LP) and a [1,2,3]-triazole spacer (TRZ) to three different support media. In addition to the agarose-based PuraBead and the polymethacrylate-type Fractogel, three new polymeric support media were introduced
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