M4528
Monoclonal Anti-MAP1b antibody produced in mouse
clone AA6, ascites fluid
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Synonym(s):
Anti-MAP1.2, Anti-MAP1X, Anti-MAP5
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
AA6, monoclonal
contains
15 mM sodium azide
species reactivity
human, feline, bovine, hamster, chicken, mouse, rat
technique(s)
microarray: suitable
western blot: 1:500 using a fresh total rat brain extract or an enriched microtubule protein preparation
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MAP1B(4131)
mouse ... Mtap1b(17755)
rat ... Map1b(29456)
General description
MAP1b is a microtubule associated protein that interacts with actin filaments. MAP1b regulates microtubule stabilization and the development of axons. It may also act as a scaffolding protein Addition of antibody to microtubule proteins before polymerization in immunoassays results in abnormally short (but otherwise morphologically normal) microtubules. Immunohistochemical staining of brain tissue with the product shows selective labeling of dendritic trees throughout the brain. Monoclonal Anti-MAP1b does not react with tubulin of other microtubule associated proteins.
Monoclonal Anti-MAP1b (MAP5) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Microtubule-associated protein (MAP1B) is the major microtubule associated protein in developing brain which changes its expression during development. In the new born rat brain, it is a major component of microtubules but in the adult its level is ten-fold lower.
Specificity
Monoclonal Anti-MAP1b antibody is specific for MAP1b in humans, mice, rats, bovines, cats, hamsters and chickens. The antibody does not cross-react with other MAPs or tubulin. Addition of the antibody to microtubule proteins before polymerization results in abnormally short (but otherwise morphologically normal) microtubules. In immunohistochemical staining of brain tissue, the antibody shows selective labeling of neurons with stronger staining of axons, dendrites and cell bodies.
Immunogen
rat brain microtubule-associated proteins (MAPs)
Application
Monoclonal Anti-MAP1b antibody produced in mouse has been used in:
- immunohistochemistry
- immunostaining
- fluorescence microscopy
Biochem/physiol Actions
Microtubule-associated protein (MAP1B) is the first MAP to appear in growing axons during development as it is present from the first emergence of the nascent axon from the cell body. Monoclonal Anti-MAP1b may be used to study MAP expression and cytological localization both in tissues and cell lines, under different developmental and environmental circumstances.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Masamitsu Shimazawa et al.
The Journal of pharmacology and experimental therapeutics, 332(2), 380-387 (2009-11-17)
We examined whether ((1S)-1-((((1S)-1-benzyl-3-cyclopropylamino-2,3-di-oxopropyl)amino)carbonyl)-3-methylbutyl)carbamic acid 5-methoxy-3-oxapentyl ester (SNJ-1945), a new orally available calpain inhibitor, might reduce retinal cell death in vivo and/or in vitro. Retinal cell damage was induced in vivo in mice by intravitreal injection of N-methyl-d-aspartate (NMDA), and
Association of microtubule-associated protein (MAP1B) with growing axons in cultured hippocampal neurons
Fischer I and Romano CG
Molecular and Cellular Neurosciences, 2(1), 39-51 (1991)
Human mesenchymal precursor cells (Stro-1+) from spinal cord injury patients improve functional recovery and tissue sparing in an acute spinal cord injury rat model
Hodgetts SI, et al.
Cell Transplantation, 22(3), 393-412 (2013)
Carolina Montenegro-Venegas et al.
Molecular biology of the cell, 21(20), 3518-3528 (2010-08-20)
Cultured neurons obtained from MAP1B-deficient mice have a delay in axon outgrowth and a reduced rate of axonal elongation compared with neurons from wild-type mice. Here we show that MAP1B deficiency results in a significant decrease in Rac1 and cdc42
Human pluripotent stem cells and derived neuroprogenitors display differential degrees of susceptibility to BH3 mimetics ABT-263, WEHI-539 and ABT-199
Garcia CP, et al.
Testing, 11(3), e0152607-e0152607 (2016)
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