M4401
Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse
clone MY-21, ascites fluid
Synonym(s):
Myosin Light Chain Antibody, Myosin Light Chain Antibody - Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse
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About This Item
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
MY-21, monoclonal
mol wt
antigen 20 kDa
contains
15 mM sodium azide
species reactivity
human, chicken, pig, bovine, rabbit
technique(s)
indirect immunofluorescence: 1:200 using human or chicken fibroblasts
western blot: suitable using denatured and reduced myosin of muscle
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
chicken ... MYLK2(396356)
human ... MYLK2(85366)
Related Categories
General description
Monoclonal Anti-Myosin (Light Chains 20 kDa) (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Myosin is a 500,000 Da protein known to interact with identical heavy chains (200,000 daltons each) and four light chains (15,000-20,000 daltons). Myosin molecules consist of two major regions: tail (rod) and head regions.
Specificity
Monoclonal Anti-Myosin reacts best with the 20,000 dalton light chains of chicken gizzard, intestine or pig stomach myosin (SDS heat denatured and reduced).
Immunogen
chicken lens membrane.
Application
Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse has been used in:
- western blot analysis
- fluorescence immunohistochemistry
- confocal microscopy
Monoclonal anti-myosin (Light Chains 20 kDa) antibody can be used in immunoblotting and western blotting.
Biochem/physiol Actions
Myosin is a cytoskeletal protein responsible for muscle contraction, muscle filament sliding and regulation of ATPase activity. Monoclonal anti-myosin (Light Chains 20 kDa) antibody diluted 1:200 can be used to detect MLC20. It is also useful in staining (1:10,000) 2D slab gels. Anti-myosin(Light Chains 20 kDa) antibody reacts specifically with 20 kDa light chain of chicken gizzard, chicken intestine and pig stomach myosin which is SDS, heat denatured and reduced but not with intact myosin molecule in its native state.
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Numerous investigators have shown that actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Myosin molecules spontaneously assemble into filaments in solutions of physiologic ionic strength and pH. Thick filaments consist mainly of myosin molecules. Myosin is an enzyme (ATPase) and its activity is the immediate source of the free energy that drives muscle contraction. Myosin binds to the polymerized form of actin, the major constituent of the thin filament.
Physical form
The product is provided as ascites fluid with 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Single Molecule Studies of Myosins
Farrow RE, et al.
Single Molecule Biology, 1-1 (2009)
Toshihito Kakei et al.
Proceedings of the Japan Academy. Series B, Physical and biological sciences, 88(5), 201-211 (2012-06-13)
Chara myosin is plant myosin responsible for cytoplasmic streaming and moves actin filaments at 60 µm/s, which is the fastest of all myosins examined. The neck of the myosin molecule has usually mechanical and regulatory roles. The neck of Chara
Contribution of increased VEGF receptors to hypoxic changes in fetal ovine carotid artery contractile proteins
Adeoye OO, et al.
American Journal of Physiology. Cell Physiology, 304(7), C656-C665 (2013)
G Redaelli et al.
Circulation research, 82(5), 594-603 (1998-04-07)
Recently, insulin-like growth factor-1 (IGF-1) has been claimed to positively influence the cardiac performance of the decompensated heart. On this basis, the effects of constitutive overexpression of IGF-1 on the mechanical behavior of myocytes were examined in transgenic mice in
Intracellular ascorbate prevents endothelial barrier permeabilization by thrombin
Parker WH, et al.
The Journal of Biological Chemistry, 290(35), 21486-21497 (2015)
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