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M4278

Sigma-Aldrich

Monoclonal Anti-MAP1 antibody produced in mouse

clone HM-1, ascites fluid

Synonym(s):

Anti-MAP1a

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About This Item

MDL number:
MFCD00164681
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

HM-1, monoclonal

contains

15 mM sodium azide

species reactivity

mouse, rat

technique(s)

immunohistochemistry (frozen sections): suitable
microarray: suitable
western blot: 1:500 using a fresh total rat brain extract or an enriched microtubule protein preparation

isotype

IgG1

UniProt accession no.

Q9QYR6

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Mtap1a(17754)
rat ... Map1a(25152)

General description

Monoclonal Anti-MAP1 (mouse IgG1 isotype) is derived from the HM-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a rat brain MAPs preparation. MAP1 is one of the major neuronal MAPs as well as being the largest (350 kD). MAP1 is more generally distributed, being found in both dendrites and axons of neurons and in glial cells in brain, in chromatophores and on both interphase and mitotic microtubules in various tissue culture cells.

Specificity

The antibody does not cross-react with other MAPs or tubulin. By immunohistochemical staining of brain tissue, the antibody shows selective labeling of neurons with stronger staining of axons than dendrites.

Immunogen

rat brain microtubule-associated proteins (MAPs)

Application

Monoclonal Anti-MAP1 has been used in :
  • immunoblotting
  • dot blot
  • immunocytochemistry
  • immunohistochemistry

Biochem/physiol Actions

Microtubules are the ubiquitous cytoskeletal structural components that are involved in intracellular transport. They are composed of tubulin and microtubule-associated proteins (MAPs). MAPs are known to mediate the binding of membranous organelles, actin filaments and intermediate filaments to microtubules. Therefore, it might be important for cellular processes such as mitosis and organelle transport, and for determining the dynamic properties of the cytoskeleton.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 1

Regulatory Information

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Drosophila Futsch/22C10 is a MAP1B-like protein required for dendritic and axonal development
Hummel T, et al.
Neuron, 26(2), 357-370 (2000)
Cytoskeletons in the Closet-Subversion in Alphaherpesvirus Infections
Denes C, et al.
Viruses, 10(2), 79-79 (2018)
The calcium-sensitive large-conductance potassium channel (BK/MAXI K) is present in the inner mitochondrial membrane of rat brain
Douglas RM, et al.
Neuroscience, 139(4), 1249-1261 (2006)
Alan Jerusalmi et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 8(6), 886-894 (2003-12-11)
We have initiated studies to determine the feasibility of employing the Semliki Forest virus (SFV) expression system as a central nervous system (CNS) vector. We investigated the effects of infecting Balb/c mice intranasally (i.n.) with recombinant SFV particles expressing the
Effect of intranasal administration of semliki forest virus recombinant particles expressing reporter and cytokine genes on the progression of experimental autoimmune encephalomyelitis
Jerusalmi A, et al.
Molecular Therapy, 8(6), 886-894 (2003)
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